HPLC and UHPLC columns
What’s in our names?
Tosoh Bioscience has the most comprehensive selection of process media resins, with a variety of pore and particle size combinations for several modes of chromatography. Here’s how you can identify the right column for your analysis:
1. Stationary Phases
Tosoh Bioscience basically uses two base materials for the (U)HPLC
columns: silica and polymer. Abbreviations used for the base matrix are SW for silica and PW for polymer. Stationary phases used with organic mobile phases for Gel Permeation Chromatography (GPC) consist of a styrene-divenylbenzene polymer and typically carry an ‘H’ in their names.
2. (U)HPLC Stationary Phase Ligands
| TSKgel ligands |
|
| Mode |
Ligand |
| HILIC |
Amide, NH2 |
| Anion Exchange |
Q, DEAE |
| Cation Exchange |
CM, SP |
| HIC |
Ether, Phenyl, Butyl |
| Reversed Phase |
CN, C1, C4, Phenyl, C8, C18 |
| Affinity |
Fc gamma IIIa receptor, Protein A, Boronate, Chelate, Tresyl |
3. Pore Size of SW-Series Columns
| Grade |
Pore size SW Series (nm) |
| G2000, SuperSW2000 |
12,5 |
| G3000, SuperSW3000, SuperSW mAb |
25 |
| UltraSW Aggregate |
30 |
| G4000 |
45 |
4. Additional Abbreviations
| We use the following abbreviations to highlight their features: |
| NPR |
non-porous |
| HTP |
High Throughput |
| HR |
High Resolution |
| AF |
Affinity |
| RP |
Reversed Phase |