Gel filtration chromatography (aqueous SEC)

TSKgel Gel Filtration Chromatography Columns

Gel Filtration Chromatography (GFC) is popular among biochemists for the separation of proteins or nucleic acid fractions, to desalt a protein sample, or to characterize water-soluble polymers used in food products, paints, and pharmaceutical preparations.

TSKgel SW-type silica-based columns, first introduced in 1977, are the biopharmaceutical industry's standard in gel filtration chromatography of biomolecules.

TSKgel PW-type polymer-based columns are the ideal choice for analyses of water-soluble polymers.

TSKgel UP-SW columns consist of varying pore sizes (from 12.5 to 30 nm), allowing for the analysis of small to very large molecular weight biopharma products.  This range of products analyzes small proteins, peptides and oligonucleotides, monoclonal antibodies, and high order mAb aggregates, high molecular weight (MW) proteins and nucleotides. These columns are compatible with HPLC and UHPLC systems and the UP-SW-LS columns are dedicated for light-scattering and mass spectrometry detection.

The TSKgel SW mAb products consist of three specialized columns designed for the analysis of monoclonal antibodies (mAbs). Compared to competitive columns, these stainless steel, silica-based TSKgel columns offer reduced lot-to-lot variation, long column life, reduction of unspecified adsorption, and superior recovery of

TSKgel SW, SWXL and SuperSW columns are silica-based columns optimized for the separation of proteins and nucleic acids using an aqueous buffer as the mobile phase. A hydrophilic diol-type bonded phase shields the silica surface from interacting with protein samples. Compared to competitive HPLC columns, TSKgel SW-type columns contain more pore volume per unit column volume, which results in higher molar mass (MM) selectivity or better resolution. The columns within the TSKgel SW-type group differ in pore size, offering the ability to separate a wide range of compounds of varying molar mass.


Column Type 
Particle Size (µm)  Benefit 
UP-SW » 2, 3 Range of pore sizes for analysis of small to large MW biopharma products; dual functionality for use with both HPLC and UHPLC systems
Super mAb » 3, 4 High resolution/high throughput analysis of monoclonal antibody monomer, fragments, and higher order mAb aggregates
SW » 10, 13, 17 High sample capacity; semi-prep dimensions available; 60 cm L available for higher resolution
SWXL » 5, 8 Most popular and most versatile; 15 cm L available for high speed; available in PEEK housing for biomolecules and ionic compounds
SuperSW » 4 4.6 mm ID for increased sensitivity and efficiency in sample-limited cases, reduced solvent consumption

The TSKgel PW columns contain particles of 12 and 17 µm and deliver high resolution separations. TSKgel PWXL columns are of smaller particle size than the TSKgel PW columns, offering higher efficiency for optimal resolution. 

The main application areas for TSKgel PW and PWXL columns are the analysis of celluloses, acrylamides, glycols, dextrans, polyvinylalcohol, and oligosaccharides. Both the TSKgel PW and PWXL column lines include a mixed bed column, prepared by mixing particles of different pore sizes, which results in columns with an extended linear calibration curve. These columns are useful as scouting columns, to quickly categorize the molar mass (MM) profile of an unknown sample. Additionally, mixed bed TSKgel PW and PWXL columns are highly useful in the characterization of samples having a very broad MM distribution.

Three specialty columns are available within the TSKgel PWXL column line. TSKgel G-DNA-PW is designed for the separation of large polynucleotides, such as DNA and RNA fragments of 500-5,000 base pairs. TSKgel G-Oligo-PW is the preferred column for the analysis of oligosaccharides. TSKgel SuperOligoPW contains smaller particles and semi-micro dimensions compared to the TSKgel G-Oligo-PW column, enabling high speed separations with high resolution of aqueous oligomers.

TSKgel PWXL-CP columns were specifically designed for the analysis of water-soluble cationic polymers. Cationic groups are introduced on the surface of the TSKgel PWXL-CP packing material to prevent undesirable interaction of cationic polymers and allow elution under low salt conditions.

TSKgel SuperMultiporePW columns are packed with particles containing a wide range of pore sizes. The unique multi-pore technology creates a linear calibration curve within each particle, resulting in better accuracy for molar mass determination.

Column Type 
Particle Size (µm)  Benefit 
PW » 12, 17  Larger particle sizes for analyses when time is not critical or sample mass is not limited; mixed bed column available for scouting purposes 
PWXL » 3, 7, 10, 13 High efficiency for optimal resolution, reduced analysis time due to smaller particle sizes; mixed bed and specialty columns available
PWXL-CP » 7, 10, 13 Improved recovery and separation of cationic polymers
SuperMultiporePW » 4, 5, 8 Unique multi-pore particles for improved linearity; three columns available, allows wide separation range from high MM polymers to oligomers