Hydroxyapatite Chromatography

Hydroxyapatite Chromatography

Ca⁺⁺Pure-HA is a hydroxyapatite resin used in the chromatographic separation of biomolecules. Key applications for use of Ca⁺⁺Pure-HA include the purification of monoclonal and polyclonal antibodies, including IgG, IgA, IgM, antibody fragments, fusion- and phosphoproteins, and the separation of single-stranded and double-stranded DNA. 

Ca⁺⁺Pure-HA resin has been sintered at high temperatures for increased mechanical and pH stability, allowing it to withstand the rigors of industrial-scale applications. Ca⁺⁺Pure-HA has a demonstrated dynamic binding capacity (DBC), at 5% breakthrough, of 55 mg mAb/mg resin at 5 minute residence time. 

Ca⁺⁺Pure-HA provides exceptional separation properties and unequalled selectivity and resolution for multiple classes of biomolecules. The robust nature of Ca⁺⁺Pure-HA offers the flexibility to use this resin at any stage in a process.

Structure of Ca++Pure-HA


VERDOT’s InPlace chromatography columns and skids

VERDOT®’s InPlaceTM chromatography columns and skids offer an ideal solution for packing, unpacking, and cleaning in place of incompressible ceramic resins. With its unique low-shear slurry valves, InPlaceTM is the perfect hardware solution for effortless and reproduceable packing of Ca++Pure-HA.

More information about the VERDOT® InPlaceTM chromatography sytems – the best-in-classs equipment for packing Ca++Pure-HA at production scale »

Product Attributes

Particle size (mean) 39 µm
Shipped as dry powder
pH stability 6.5 - 14.0
Stability data 10 years

More information on stabillity and dynamic binding capacity for Ca++Pure-HA  »

Ordering Information

P/N Description Dimension
0045045 Ca++Pure-HA 50 g
0045039 Ca++Pure-HA 100 g
0045040 Ca++Pure-HA 250 g
0045041 Ca++Pure-HA 500 g
0045042 Ca++Pure-HA 1 kg
0045043 Ca++Pure-HA 5 kg
0045231 SkillPak Mixed Mode 1 mL col. library
(Ca++Pure-HA, MX-Trp-650M 1mL x 3 ea)
7 mm ID x 2.5 cm L
0045232 SkillPak Best in Class 1 mL col. Library
(AF-rProtein A HC-650F, AF-rProtein L-650F, NH2-750F,
Sulfate-650F, Ca++Pure-HA 1 mL x 1 ea)
7 mm ID x 2.5 cm L
0045225 SkillPak Ca++Pure-HA 5 x 1 mL col. 7 mm ID x 2.5 cm L
0045265 SkillPak Mixed Mode 5 mL col. Library
(Ca++Pure-HA, MX-Trp-650M 5 mL x 1 ea)
8 mm ID x 10 cm L
0045266 SkillPak Best in Class 5 mL col. library
(AF-rProtein A HC-650F, AF-rProtein L-650F, NH2-750F,
Sulfate-650F, Ca++Pure-HA 5 mL x 1 ea)
8 mm ID x 10 cm L
0045262 SkillPak Ca++Pure-HA 5 mL col. 8 mm ID x 10 cm L
0045053 RoboColumn Ca++Pure-HA 200 µL
0045054 RoboColumn Ca++Pure-HA 600 µL
0045512 Resin Seeker Ca++Pure-HA 20 µL
0045513 Resin Seeker Ca++Pure-HA 500 µL