TOYOPEARL Phenyl-650
TOYOPEARL Phenyl-650 is based on a 100 nm (1000 Å) pore size polymethacrylate base material bonded with phenyl groups. This resin has an intermediate hydrophobicity. Primary applications are for the separation of proteins, their isoforms, and aggregate removal. It is typically used in intermediate purification and polishing steps. For high resolution separations, the use of TSKgel Phenyl-5PW media is suggested. Both products have the same selectivity.
TOYOPEARL Phenyl-650 is available in three particle sizes, S-, M-, and C-grade. This resin is also available in pre-packed SkillPak columns to simplify your process development and scale up.
Structure of TOYOPEARL Phenyl-650 resins
|
|
Product attributes
| Pore size (mean): |
100 nm |
| Particle size (mean): |
100 µm (C-grade)
65 µm (M-grade)
35 µm (S-grade) |
| Shipping buffer: |
20% ethanol |
| pH stability: |
>1-13 |
| Stability data: |
10 years (estimated) |
Typical dynamic binding capacities for humanized mAb
|
 |
Binding capacitiy (mg/mL - gel)
(10% breakthrough)
Phenyl-650M 35.2
Butyl-650M 42.1
PPG-600M 44.8
Butyl-600M 48.5 |
Selectivity of TOYOPEARL HIC resins
|
 |
| Samples: 1. Ribonuclease A, 2. Lysozyme, 3. α- Chymotrypsinogen |