TSKgel ODS-120A
TSKgel ODS-120A columns are based on a 15 nm pore size silica base support bonded with ODS groups. The bonding method results in a polymeric coverage of C18 groups on the silica surface and an effective pore size of 12 nm (120 Å). TSKgel ODS-120A is not endcapped. The silica base support’s exclusion limit of 1.0 × 10^4 Da makes them a good choice for the reversed phase chromatography of peptides and small proteins.
TSKgel ODS-120A structure |
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Separation of 16 poly-aromatic hydrocarbons |
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Column: TSKgel ODS-120A, 4.6 mm ID x 25 cm
Gradient: 40 min linear from 75% MeOH/25% H2O to 95% MeOH/5% H2O,
5min hold
Flow rate: 1.2 mL/min
Detection: UV @ 254 nm
Temperature: 40°C
Sample: 5 mL mixture of:
1. Naphthalene, 2. Acenaphthylene
3. Acenaphthene, 4. Fluorene,
5. Phenanthrene, 6. Anthracene,
7. Fluoranthene, 8. Pyrene,
9. Benzo(a)anthracene, 10. Chrysene,
11. Benzo(b)fluoranthene, 12. Benzo(k)fluoranthene,
13. Benzo(a)pyrene, 14. Dibenzo(a,h)anthracene,
15. Benzo(g,h,i)perylene, 16. Indeno(1,2,3-cd)pyrene |