SOLUTIONS

TSKgel SuperQ-5PW

TSKgel SuperQ-5PW

TSKgel SuperQ-5PW columns are packed with porous hydrophilic polymer beads which are modified with a strong anion exchange group. The resulting product exhibits very high protein capacities. TSKgel SuperQ-5PW columns are used for the separation and analysis of proteins, oligonucleotides and other biologically active molecules.

Stability of TSKgel SuperQ-5PW columns 

superq-5pw_fig1.png 
Column:           TSKgel SuperQ-5PW, 10 µm, 7.5 mm ID × 7.5 cm
Mobile phase:  A: 50 mmol/L Tris-HCI, pH 8.6
                          B: 0.5 mmol/L sodium cloride in 50 mmol/L Tris-HCI,
                          pH 8.6
Gradient:        A-B (60 min)
Flow rate:       1.0 mL/min
Detection:       UV @ 280 nm
Temperature: 25 °C
Injection vol.: 100 µL
Sample load:   each of 1 mg
Samples:         1. ovalbumin, 2. trypsin inhibitor
Note:               A: before CIP, B: after 15 times (15 days) 

 

Analysis of 16-mer oligonucleotide 

superq-5pw_fig2.png 
Column:          TSKgel SuperQ-5PW, 10 µm, 7.5 mm ID × 7.5 cm
Mobile phase: A: 10 mmol/L NaOH
                         B: 10 mmol/L NaOH with 1 mol/L NaCl
Gradient:        0 min (0%B) 40 min (50%B)
                         41 min (100%B) 46 min (100%B)
Flow Rate:      1 mL/min
Detection:       UV @ 254 nm
Sample:          16-mer morpholine oligonucleotide,
                        AAG AAG AAG AGG GGA G
Sample load:  0.5 O.D. (optical density) 

 

Comparison of various anion exchange columns under large sample load 

superq-5pw_fig3.png 
Columns:        A: TSKgel SuperQ-5PW, 10 µm, 5 mm ID × 5 cm
                         B: Company A, Q type, 5 mm ID × 5 cm
                         C: Company A, perfusion Q type, 6.4 mm ID × 3 cm
                         All column volumes were 1.0 mL
Mobile phase: A: 50 mmol/L Tris-HCl buffer, pH 8.3
                         B: A + 0.5 mol/L NaCl
                         A to B linear gradient (60 min)
Flow rate:       0.8 mL/min
Detection:       UV @ 280 nm
Temperature: 25 °C
Injection vol.: 2 mL
Samples:         ovalbumin, 20 mg
                         trypsin inhibitor, 20 mg 

 

Ordering Information

P/N Description Dimension
0018386 TSKgel SuperQ-5PW, Glass 8 mm ID x 7.5 cm L
0018257 TSKgel SuperQ-5PW 7.5 mm ID x 7.5 cm L
0018387 TSKgel SuperQ-5PW 21.5 mm ID x 15 cm L
0018388 TSKgel SuperQ-5PW Guardgel Kit for P/N 0018257