TSKgel SP-NPR
The TSKgel SP-NPR column is packed with 2.5 µm, nonporous hydrophilic polymer beads of which the surface has been modified with a strong cation exchange group. Because this column contains non-porous particles, binding capacity is low compared to porous columns with the same ligand functionality, whereas protein recovery and efficiency is generally very high.
The TSKgel SP-NPR column is used for the separation and analysis of proteins and peptides. This column is particularly useful for adeno-associated viruses and other large biopolymers.
Analysis of purified AAV |
 |
Column: TSKgel SP-NPR, 2.5 µm, 4.6 mm ID × 3.5 cm
Mobile phase: A. 50 mmol/L HEPES, 1 mmol/L EDTA, 5 mmol/L MgCl, pH 7.5
B. 50 mmol/L HEPES, 1 mmol/L EDTA, 5 mmol/L MgCl,
pH 7.5 with 0.5 mol/L NaCl; linear gradient from 20% to 100%B
in 10 column volumes
Gradient: 0 min (0%B) 2 min (100%B)
Flow rate: 1 mL/min
Detection: UV @ 280 nm
Sample: purified adeno-associated virus |
pH gradient analysis of hemoglobin A1c |
 |
Column: TSKgel SP-NPR, 2.5 µm, 4. 6 mm ID × 3.5 cm
Mobile phase: A: 0.02 mol/L MES, and 0.02 mol/L HEPES-NaOH, pH 6.0
B: 0.02 mol/L MES, and 0.02 mol/L HEPES- NaOH, pH 8.0
Gradient: 10 min linear gradient from 32% to 75% buffer B
(pH 6.66 to pH 7.43)
Flow rate: 1.5 mL/min
Detection: VIS @ 415 nm
Sample: hemoglobin standard |