SOLUTIONS

TSKgel DNA-NPR

TSKgel DNA-NPR

The TSKgel DNA-NPR column is packed with 2.5 µm, nonporous, hydrophilic polymer beads which are surface modified with a weak anion exchange group. Because this column contains non-porous particles, binding capacity is low compared to porous columns with the same ligand functionality, whereas protein recovery and efficiency is generally very high.

The TSKgel DNA-NPR column dimensions are optimized for the high efficiency separation of DNA fragments, PCR products, or plasmids.

Plasmid Analysis 

dnanpr_fig1.png 
Column:          TSKgel DNA-NPR, 2.5 µm, 4. 6 mm ID × 7.5 cm
Mobile phase: A. 20 mmol/L Tris, pH 9.0
                        B. 20 mmol/L Tris, pH 9.0 with 1 mol/L NaCl
Gradient:         linear gradient from 50% to 65%B in 10 column volumes
Flow rate:       1 mL/min
Detection:       UV @ 260 nm
Sample:          PUC19 plasmid 

 

Plasmid Analysis 

dnanpr_fig2.png 
Column:          TSKgel DNA-NPR, 2.5 µm, 4.6 mm ID × 7.5 cm
Mobile phase: A: 10 mmol/L sodium bromide, 20 mmol/L NaOH,
                         pH 12, 1% diethylamine
                         B: 1 mol/L sodium bromide, 20 mmol/L NaOH,
                         pH 12, 1% diethylamine
Gradient:        3.5 min (20%B) 12 min (20%B) 45 min (55%B)
Flow rate:       1.0 mL/min
Temperatures: 60 °C (column), 4 °C (sample chamber)
Sample:           crude deprotected 13-mer oligonucleotide 

 

Ordering Information

P/N Description Dimension
0018249 TSKgel DNA-NPR 4.6 mm ID x 7.5 cm L
0018253 TSKgel DNA-NPR Guard Column
for P/N 0018249
4.6 mm ID x 0.5 cm L