SOLUTIONS

TSKgel DEAE-NPR

TSKgel DEAE-NPR 

The TSKgel DEAE-NPR column is packed with 2.5 µm, nonporous, hydrophilic polymer beads which are surface modified with a weak anion exchange group. These columns are used for the high speed separation and analysis of proteins and poly- and oligonucleotides. TSKgel DEAE-NPR columns are particularly useful for high resolution separation of DNA digests or fragments.

Analysis of DNA digest  

deaenpr_fig1.png 
Column:          TSKgel DEAE-NPR, 2.5 µm, 4. 6 mm ID × 3.5 cm,
                         with guard column, 4.6 mm ID × 0.5 cm
Mobile phase: A: 0.02 mol/L Tris-HCI, pH 9.0
                         B: mobile phase A plus 1.0 mol/L NaCl
Gradient:        15 min linear gradient from 48% to 65% mobile phase B
Flow rate:       1.5 mL/min
Detection:       UV @ 260 nm
Pressure:        14 MPa
Temperature: 40 ºC
Sample:          Hae III digest of pBR322 DNA,
                        (base pair number for each peak is indicated) 

 

Detection of HIV-1 PCR-Amplified 130 bp target 

deaenpr_fig2.png 
Column:           TSKgel DEAE-NPR, 2.5 µm, 4.6 mm ID × 3.5 cm
Mobile phase:  A: 20 mmol/L Tris-HCl with 0.25 mol/L NaCl, pH 7.7
                          B: 20 mmol/L Tris-HCl with 1 mol/L NaCl, pH 7.7
Flow rate:        1 mL/min
Detection:        UV @ 260 nm
Temperature:  ambient
Sample:            HIV-1 PCR-amplified 130 bp target
Sample load:   20 µL 

 

Ordering Information

P/N Description Dimension
0013075 TSKgel DEAE-NPR 4.6 mm ID x 3.5 cm L
0017088 TSKgel Guard Column for P/N 0013075, 5 µm 4.6 mm ID x 0.5 cm L