SOLUTIONS

TSKgel DEAE-5PW

TSKgel DEAE-5PW

TSKgel DEAE-5PW columns are packed with porous, hydrophilic polymer beads which are surface modified with a weak anion exchange group. These columns are used for the separation and analysis of proteins, along with nucleotides, nucleosides, and other biomolecules.

TSKgel DEAE-5PW columns are available in internal diameters varying from 2 mm to 21.5 mm and in column housings of either glass or stainless steel.


Analysis of high MM RNA  

deae5pw_fig1.png 
Column:          TSKgel DEAE-5PW, 10 µm, 6 mm ID × 15 cm (custom)
Mobile phase: 300 min linear gradient from 0.3 mol/L to
                         1.0 mol/L NaCl in 0.1 mol/L Tris-HCl, pH 7.6
Flow rate:       1.0 mL/min
Detection:       UV @ 260 nm
Sample:          total E. coli RNA 

 

 Detection of HIV-1 PCR-Amplified 130 bp target

 deae5pw_fig2.png
Column:          TSKgel DEAE-5PW, 10 µm, 7.5 mm ID × 7.5 cm
Mobile phase: A: 25 mmol/L Tris-HCl, 1 mmol/L EDTA, pH 8.0
                         B: 25 mmol/L Tris-HCl, 1 mmol/L EDTA, pH 8.0,
                         with 1 mol/L NaCl
Gradient:        25-60%B in 30 minutes
Flow rate:       1 mL/min
Detection:       UV @ 260 nm
Temperature: 25 °C
Sample:           pBR322 plasmid
Sample load:  2.5 mg in 1 mL

Separation of monoclonal antibody 

deae5pw_fig3.png 
Column:          TSKgel DEAE-5PW, 10 µm, 7.5 mm ID × 7.5 cm
Mobile phase: A: 20 mmol/L Tris-HCI, pH 8.5
                         B: A + 0.5 mol/L NaCl
                         A to B linear gradient (60 min)
Flow rate:       1.0 mL/min
Detection:       UV @ 280 nm
Temperature: 25 ºC
Sample:           anti-human albumin (lgG1),
                         diluted solution of mouse ascites (168 µg in 40 µL)

 

Ordering Information

P/N Description Dimension
0013061 TSKgel DEAE-5PW, Glass  5 mm ID x 5 cm L 
0008802 TSKgel DEAE-5PW, Glass 8 mm ID x 7.5 cm L
0014016 TSKgel DEAE-5PW, Glass 20 mm ID x 15 cm L
0018757 TSKgel DEAE-5PW 2 mm ID x 7.5 cm L
0007164 TSKgel DEAE-5PW 7.5 mm ID x 7.5 cm L
0007574 TSKgel DEAE-5PW 21.5 mm ID x 15 cm L
0007210 TSKgel DEAE-5PW Guardgel Kit for P/N 0007164
0016092 TSKgel DEAE-5PW Prep Guardgel Kit for P/N 0007574
0019308 TSKgel Cartridge Holder for P/N 0042152 2 mm ID x 1 cm L
0014466 TSKgel DEAE-5PW Glass Guard Column for P/N 0014016 20 mm ID x 1 cm L
0008806 TSKgel DEAE-5PW Glass Guardgel Kit for P/N 0013061, 0008802