SOLUTIONS

TSKgel DEAE-2SW

TSKgel DEAE-2SW 

TSKgel DEAE-2SW columns are packed with 5 µm, porous, spherical silica beads which are surface modified with a weak anion exchange group. They are used for the separation and analysis of nucleotides, nucleosides, peptides, small proteins and other biologically active molecules. A 2 mm ID column is available for applications that are sample limited.

Separation of nucleotides  

 deae2sw_fig1.png
Column:          TSKgel DEAE-2SW, 5 µm, 4.6 mm ID × 25 cm
Mobile phase: A: CH3CN in 0.1 mol/L phosphate, pH 3.0, 20/80
                         B: CH3CN in 0.5 mol/L phosphate, pH 3.0, 20/80
Gradient:        30 min linear gradient from buffer A to B
Flow rate:       1.0 mL/min
Detection:       UV @ 260 nm
Samples:         1. AMP 2. IMP 3. GMP 4. ADP 5. ATP

 

Separation of phosphorothioates 

 deae2sw_fig2.png
Column:          TSKgel DEAE-2SW, 5 µm, 4.6 mm ID × 25 cm
Mobile phase: A: 50 mmol/L ammonium acetate
                         B: 1.5 mol/L ammonium acetate
Gradient:        linear, 0-100%B in 60 minutes
Flow rate:       1 mL/min
Detection:       UV @ 254 nm
Temperature: 25 °C
Samples:         1. 2 base phosphorothioate oligonucleotide
                         2. 3 base phosphorothioate oligonucleotide
                         3. 4 base phosphorothioate oligonucleotide
                         4. 5 base phosphorothioate oligonucleotide 

 

Ordering Information

P/N Description Dimension
0018761 TSKgel DEAE-2SW 2 mm ID x 25 cm L
0007168 TSKgel DEAE-2SW 4.6 mm ID x 25 cm L
0007648 TSKgel DEAE-SW Guardgel Kit