TSKgel CM-5PW
TSKgel CM-5PW columns are packed with porous hydrophilic polymer beads which are surface modified with carboxymethyl (CM) ligands to provide a weak cation exchange column. They are used for the separation and analysis of proteins, peptides, and other biologically active molecules.
Selectivity of strong and weak TSKgel cation exchange columns
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Columns: TSKgel SP-5PW and TSKgel CM-5PW, 10 µm, 7.5 mm ID × 7.5 cm
Mobile phase: 60 min linear gradient from 0 mol/L to 0.5 mol/L NaCl
in 0.02 mol/L phosphate, pH 7.0
Flow rate: 1.0 mL/min
Detection: UV @ 280 nm
Samples: 1. trypsinogen, 2. ribonuclease, 3. α-chymotrypsinogen
4. cytochrome C, 5. lysozyme |