SOLUTIONS

TSKgel BioAssist Q

TSKgel BioAssist Q 

TSKgel BioAssist Q columns are packed with polymethacrylate particles containing very large pores (~400 nm) that are functionalized with polyamine groups to form a network structure. The very large pores allow large biomolecules to freely enter and exit the pores resulting in higher binding capacity and better recovery of activity than other competitive materials. The capacity of the TSKgel BioAssist Q columns is high over a wide molecular weight range (up to 1.0 × 10^6 Da).

The standard TSKgel BioAssist Q column is 4.6 mm ID x 5 cm, while a 10 mm ID x 10 cm semi-preparative column is available for scale-up. Both columns are made of PEEK to reduce protein adsorption.

Analysis of mouse ascites fluid

 bioassistq_fig1.png

Columns:        A: TSKgel BioAssist Q, 10 µm, 4.6 mm ID × 5 cm 
                         B: Commercial Q-type product A, 5.0 mm ID × 5 cm
Mobile phase: 15 min linear gradient of NaCl from 0 to 1.0 mol/L
                         in 20 mmol/L Tris-HCl buffer, pH 8.0
Flow rate:       1.0 mL/min
Detection:       UV @ 280 nm 
Temperature: 25 ºC
Injection vol.: 5 µL
Sample:          mouse ascites fluid (3-fold dilution with initial eluent)

 Performance enhancement

bioassistq_fig2.png

Columns:        A: TSKgel BioAssist Q, 10 µm, 4.6 mm × 5 cm 
                         B: Competitor Q, 5.0 mm ID × 5 cm
Mobile phase: 30 min linear gradient from 0 to 1 mol/L NaCl
                         in 20 mmol/L sodium phosphate, pH 8.0
Flow rate:       1.0 mL/min 
Detection:       UV @ 280 nm 
Samples:         1. conalbumin 
                         i. ovalbumin impurity
                         2. ovalbumin
                         3. trypsin inhibitor 

Dynamic binding capacity as function of protein molar mass 

 bioassistq_fig3.png
Columns:        TSKgel BioAssist Q, 10 µm, 4.6 mm ID × 1 cm 
                         Conventional Q-type product A, 5.0 mm ID × 1 cm
                         TSKgel SuperQ-5PW, 4.6 mm ID × 1 cm
Mobile phase: 20 mmol/L Tris-HCI buffer, pH 8.0
Flow rate:        0.38 mL/min
Detection:        UV @ 280 nm 
Temperature:  25 ºC
Samples:          1. trypsin inhibitor, 10 g/L
                          2. human serum albumin, 10 g/L
                          3. lgG1, 2.3 g/L
                          4. thyroglobulin, 5 g/L

 

 

Ordering Information

P/N Description Dimension
0019685 TSKgel BioAssist Q, Peek 4.6 mm ID x 5 cm L
0021410 TSKgel BioAssist Q, Peek 10 mm ID x 10 cm L