TOYOPEARL SP-550 is a strong cation exchange resin for biomolecule purification. It is composed of polymethacrylate beads that have been functionalized with Sulfopropyl (SP) strong cation exchange groups. TOYOPEARL SP-550 has high chemical and mechanical stability, allowing cleaning-in-place (CIP) and very high flow rates.

TOYOPEARL SP-550 is available 100 µm particle size (C-grade), designed to be used for high throughput capture of product from biological feedstock. It exhibits typical dynamic binding capacities for lysozyme of 100 g/L. This resin is also available in pre-packed SkillPak columns to simplify your process development and scale up.

Structure of TOYOPEARL SP-550  



Product attributes

Pore size (mean): < 50 nm
Particle size (mean): 100 µm (C-grade)
Shipping buffer: 20% ethanol
pH stability: 2-13
Stability data: 10 years (estimated)

Breakthrough curves of mono-PEGylated lysozyme using TOYOPEARL cation exchange resins 

Resins:            A. TOYOPEARL SP-650M B. TOYOPEARL CM-650M
                         C. TOYOPEARL SP-550C D. TOYOPEARL GigaCap CM-650M
                         E. TOYOPEARL GigaCap S-650M
Column size:   6 mm ID × 40 mm
Mobile phase: Buffer A: 20 mmol/L phosphate buffer, pH 7.0
                         Buffer B: 20 mmol/L phosphate buffer, pH 7.0 + 0.5 mol/L NaCl
Flow rate:        212 cm/hr (1.0 mL/min)
Detection:        UV @ 280 nm
Sample:           mono-PEGylated lysozyme, 1.0 mg/mL (PEG MW= 5 kDa)

Dynamic binding capacities were determined at 10% breakthrough 


Ordering Information

P/N Description Dimension
0043272 TOYOPEARL SP-550C 100 mL
0014028 TOYOPEARL SP-550C 250 mL
0014705 TOYOPEARL SP-550C 1 L
0014029 TOYOPEARL SP-550C 5 L
0045213 SkillPak 1 SP-550C 5 x 1 mL col. 7 mm ID x 2.5 cm L
0045249 SkillPak 5 SP-550C 5 mL col. 8 mm ID x 10 cm L
0045322 SkillPak 50 SP-550C 50 mL col. 2.5 cm ID x 10 cm L
0045323 SkillPak 200 SP-550C 200 mL col. 5 cm ID x 10 cm L