TOYOPEARL GigaCap S-650
TOYOPEARL GigaCap S-650 media are high capacity, high resolution cation exchange resins optimized for the capture and purification of both small and large proteins, including monoclonal antibodies. They are composed of polymethacrylate beads that have been chemically modified to provide a higher number of cationic binding sites and functionalized with sulfopropyl (S) strong cation exchange groups.
TOYOPEARL GigaCap S-650 resins can be used for high throughput capture, intermediate purification, and polishing process steps. They are available in two particle sizes: 75 µm (M-grade), and 35 µm (S-grade). TOYOPEARL GigaCap S-650 media also have excellent binding and elution kinetics, which results in a more concentrated protein fraction collected during the elution step.
TOYOPEARL GigaCap S-650M exhibits typical dynamic binding capacities greater than 150 g/L for human IgG and maintains its high capacity at increased linear velocities.
TOYOPEARL GigaCap S-650S has a dynamic binding capacity greater than 160 g/L for human IgG. This high capacity, combined with the 35 µm mean particle size, makes TOYOPEARL GigaCap S-650S ideal for high resolution applications.
This resin is also available in pre-packed SkillPak columns to simplify your process development and scale up.
Structure of TOYOPEARL GigaCap S-650M
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Product attributes
| Pore size (mean): |
< 100 nm |
| Particle size (mean): |
75 µm (M-grade)
35 µm (S-grade) |
| Shipping buffer: |
20% ethanol |
| pH stability: |
2-13 |
| Stability data: |
10 years (estimated) |
TOYOPEARL GigaCap S-650M vs. Competitor S elution pool volume comparison
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Column size: 6 mm ID x 40 mm bed
Sample: polyclonal human IgG (1 mg/mL)
Loading Buffer: 0.1 mol/L acetate buffer (pH= 4.7)
Elution Buffer: 0.1 mol/L acetate buffer (pH= 4.7) + 1.0 mol/L NaCl
Linear velocity: 212 cm/h
Detection: UV @ 280 nm |
TOYOPEARL GigaCap S-650M human IgG breakthrough curves at various linear velocities
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Resins: TOYOPEARL GigaCap S-650M
Column size: 6 mm ID × 4 cm (1.13 mL)
Mobile phase: 0.1 mol/L acetate buffer, pH 4.7
Flow rates: 212, 424, 636, 1060 cm/hr (1.0, 2.0, 3.0, 5.0 mL/min)
Detection: UV @ 280 nm
Sample: polyclonal human IgG (1 g/L) |
Selectivity comparison between native protein and mono-PEGylated protein on TOYOPEARL GigaCap resins
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Resins: TOYOPEARL GigaCap S-650M TOYOPEARL GigaCap CM-650M
Column size: 6 mm ID × 4 cm
Mobile phase: Buffer A: 50 mmol/L phosphate buffer, pH 7.0
Buffer B: 50 mmol/L phosphate buffer, pH 7.0 + 0.5 mol/L NaCl
Gradients: 10 minute 100% buffer A 10 minute 100% buffer A 60 minutes
0%B to 100%B 60 minutes 0%B to 50%B
Flow rate: 212 cm/hr (1 mL/min)
Samples: native lysozyme, 5 g/L mono-PEGylated lysozyme, 5 g/L
(PEG MW= 5 kDa) |