TSKgel UltraSW Aggregate
The TSKgel UltraSW Aggregate column is designed with a higher exclusion limit and smaller particle size (3 µm) than the other TSKgel SW mAb columns and is ideally suited for the high resolution analysis of higher order mAb aggregates.
The larger molecular weight exclusion limit of the TSKgel UltraSW Aggregate column allows for superior resolving power for multimers and aggregates of large proteins, including thyroglobulin and IgG.
It is important to employ an UHPLC system or an HPLC system that is optimized with regards to extra-column void volume to take full advantage of the high efficiency that can be obtained on TSKgel UltraSW Aggregate.
Separation of mAb trimer and dimer |
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Column: TSKgel UltraSW Aggregate, 3 µm, 7.8 mm ID × 30 cm
Mobile phase: 0.2 mol/L phosphate buffer, pH 6.7 + 0.05% NaN3
Flow rate: 0.8 mL/min
Detection: UV @ 280 nm
Temperature: 25 ˚C
Sample: monoclonal antibody-2 (mouse-human chimeric IgG, Erbitux), 10 µL |
Analysis of heat induced forced denatured, large hydrophobic metalloprotein, apoferritin |
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Column: TSKgel UltraSW Aggregate, 3 μm, 7.8 mm ID × 30 cm
Mobile phase: 50 mmol/L potassium phosphate (monobasic),
50 mmol/L sodium phosphate (dibasic),
100 mmol/L sodium sulfate, 0.05% NaN3, pH 6.7
Flow rate: 1.0 mL/min
Detection: UV @ 280 nm
Temperature: 30 °C
Injection vol.: 10 μL
Samples: ferritin – Sigma, 4.7 g/L, in saline (0.9% NaCl in water) solution, stored at
2-8 °C apoferritin – Sigma, 5.0 g/L, in 50% glycerol and 0.075 mol/L
sodium chloride, stored at -20 °C |