TSKgel SuperSW mAb HR
The TSKgel SuperSW mAb HR gel filtration column offers high resolution analysis of mAb fragments, monomer, and dimer. The high resolution capability of this column is due to a unique pore-controlled technology, which produces a shallow calibration curve in the molecular weight region of a typical monoclonal antibody.
Separation of human IgG monomer, half-body, and fragments using a TSKgel SuperSW mAb HR column |
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Column: TSKgel SuperSW mAb HR, 4 μm, 7.8 mm ID × 30 cm
Mobile phase: 0.1 mol/L phosphate/0.1 mol/L sulfate buffer + 0.05% NaN3
Flow rate: 0.5 mL/min
Detection: UV @ 280 nm
Temperature: 25 ºC
Injection vol.: 10 μL
Sample: human IgG (4.6 g/L) from Sigma Aldrich |
Comparison of resolution of mAb monomer and dimer |
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Columns: A. TSKgel SuperSW mAb HR, 4 µm, 7.8 mm ID × 30 cm
B. Brand A, 3 μm, 7.8 mm ID × 30 cm
C. Brand B, 5 μm, 7.8 mm ID × 30 cm
D. Brand C, 5 μm, 8.0 mm ID × 30 cm
E. Brand D, 5 μm, 8.0 mm ID × 30 cm
Mobile phase: 200 mmol/L phosphate buffer, pH 6.7 + 0.05% NaN3
Flow rate: 1.0 mL/min
Detection: UV @ 280 nm
Temperature: 25 ˚C
Injection vol.: 10 μL
Sample: IgG (human polyclonal), 1.0 g/L
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High durability of TSKgel SuperSW mAb HR column |
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Column: TSKgel SuperSW mAb HR, 4 µm, 7.8 mm ID × 30 cm
Mobile phase: 0.2 mol/L phosphate buffer, pH 6.7 + 0.05% NaN3
Flow rate: 0.8 mL/min
Detection: UV @ 280 nm
Injection vol.: 10 μL
Samples: 1. g-Globulin
2. Cytochrome C
3. DNP-L-Alanine |