TSKgel SuperSW3000
TSKgel SuperSW3000 are packed with ultra-efficient 4 μm particles, providing high efficiency separations. The 25 nm pore size results in a fractionation range up to 500,000 Da for globular proteins. The narrow 4.6 mm column inner diameter reduces buffer consumption and is ideal for sample-limited applications.
It is important to employ an HPLC system that is optimized with regards to extra-column band broadening to take full advantage of the high column efficiency that can be obtained on TSKgel SuperSW columns.
Overlays of monoclonal antibody separation |
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Column: TSKgel SuperSW3000, 4 µm, 4.6 mm ID × 30 cm
Mobile phase: A: 0.4 mol/L NaClO4, 0.05 mol/L NaH2PO4,
B: 0.2 mol/L NaClO4, 0.05 mol/L NaH2PO4,
C: 0.2 mol/L NaCl, 0.1 mol/L NaH2PO4
Flow rate: 0.35 mL/min
Detection: UV @ 214 nm
Injection vol.: 5 µL
Samples: monoclonal antibodies |