A perfect match for biomolecule analysis

High resolution SEC columns and high sensitivity MALS detector 

Characterizing biomolecules can be challenging when sample components are related and thus similar or when analysis of the native molecule is required. The combination of UHPLC size exclusion with multi-angle light scattering analyzes biomolecules such as antibodies, mAb fragments, proteins, nucleic acids and delivery particles in a native state and provides size and molecular weight of sample components. The high resolution of TSKgel® UP-SW columns reliably separates sample components while the LenS3™ MALS detector reveals these even at the lowest concentrations and determines their size and weight.

Molecular weight distribution of an unpurified 20mer oligonucleotide

Column: TSKgel UP-SW2000, 4.6 x 30 cm L Instrument UHPLC Thermo Fisher Dionex Ultimate® 3000 UHPLC system with LenS3 MALS Mobile phase: 0.5 mol/L NaCl, 0.1 mol/L EDTA, pH 7.5 0.1 mol/L Na2SO4, 0.03% NaN3 in 0.1mol/L phosphate buffer Flow rate: 0.3 mL/min Detection: UV @ 260 nm and MALS Injection vol.: 10 µl Sample: 20 bases custom oligonucleotide with MW= 6141 Da (crude sample 1 mg/mL)

Light scattering signal (RALS) of Herceptin® biosimilar monomer, fragment and aggregates with decreasing injected mass

Column: TSKgel UP-SW3000, 4.6 x 30 cm L Instrument UHPLC Thermo Fisher Dionex Ultimate® 3000 UHPLC system with LenS3 MALS Mobile phase: 100 mmol/L NaH2PO4, pH 6.8 + 100 mmol/L Na2SO4 Flow rate: 0.35 mL/min Detection: UV @ 280 nm and MALS Injection vol.: 10 µl Sample: Herceptin biosimilar 500 ng – 2 n

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TSKgel UP-SW UHPLC size exclusion columns 

TSKgel UP-SW columns with 2 (3) µm particles, is the latest addition to the renowned TSKgel SW series of silica based gel filtration columns. Over 50 years of experience in SEC are built into the new series of UHPLC columns for the analysis of proteins in the range of 1 to 2000 kDa.

LenS3 Multi-Angle Light Scattering Detector

A Revolutionary Technology for Macromolecular Characterization

The LenS3 Multi-Angle Light Scattering Detector offers a revolutionary approach for the measurement of molecular weight (MW) and radius of gyration (R_g) of synthetic polymers, polysaccharides, proteins, and biopolymers. LenS3 is accompanied by SECview Software, offering seamless versatility from data acquisition to processing.

Highlights