sec
SEC
23
Columns for Specific Applications
TSKgel PW
XL
-CP
The new TSKgel PW
XL
-CP columns are designed to facilitate the
separation of cationic polymers by SEC at low salt conditions. They
are based on the well known PW-type of polymeric resins for aqueous
SEC. Cationic surface modification enables low salt elution of cationic
polymers with high recoveries. The columns show high theoretical
plate numbers, linear calibration curves and high durability. They are
produced with three pore sizes for diffrent ranges (G3000-, G5000- and
G6000PW
XL
-CP).
FIgure 16
shows the analysis of various cationic
polymers on a series of TSKgel PW
XL
-CP columns.
TSKgel SuperOligoPW & G-Oligo-PW
The new TSKgel SuperOligoPW column was developed for the fast
determination of molecular mass of aqueous oligomers, particularly
oligosaccharides, and low molecular weight aqueous polymers. This
is a semi-micro column (6.0 mm ID x 15 cm L) packed with spherical
monodisperse polymethacrylate 3 μm particles. The combination of
the decreased particle size and small dimensions of the TSKgel
SuperOligoPW column enables high speed separation with high
resolution - half of the separation time with the same resolution
compared to conventional size exclusion columns. An added benefit
of the semi-micro and small particle size is lower solvent consumption
compared to conventional columns.
Columns: TSKgel G3000PW
XL
-CP, 7 μm (7.8 mm ID x 30 cm L), TSKgel
G5000PW
XL
-CP, 10 μm (7.8 mm ID x 30 cm L), TSKgel G6000PW
XL
-CP, 13
μm (7.8 mm ID x 30 cm L); Eluent: 0.1 mol/L NaNO
3
; Flow rate: 1 mL/min;
Detection: RI; Temperature: 25°C; Sample Load: 3 g/L, 100 μL
TSKgel G-Oligo-PW was designed for high resolution separations
of nonionic and cationic oligomers and oligosaccharides such as
hydrolyzed cyclodextrins. Because of the presence of residual cationic
groups, this column is not recommended for separating anionic
materials. The polyethylene glycol and polythylene oxide calibration
curves for TSKgel G-Oligo-PW (not shown) are identical to the
calibration curve for TSKgel G2500PW
XL
(shown on the previous page.
Figure 18
shows the calibration curve for double stranded DNA for the
TSKgel G-DNA-PW column.
TSKgel G-DNA-PW
The TSKgel G-DNA-PW column is dedicated to the separation of large
polynucleotides, such as DNA and RNA fragments of 500 to 5,000 base
pairs. The exclusion limits for double-stranded DNA fragments are
lower than those for rRNAs. The packing of the TSKgel G-DNA-PW
column has very large pores (>100 nm) and a small particle size (10 µm).
For the separation of large DNA fragments greater than 1,000 base
pairs, a four-column system is typically required. Baseline resolution of
DNA fragments up to 7,000 base pairs can be achieved, provided there
is a two-fold difference in the chain length of the fragments.
Column: TSKgel G-Oligo-PW, two 6 μm, 7.8 mm ID x 30 cm L columns in
series; Mobile phase: distilled H
2
O; Flow rate: 1.0 mL/min;
Detection: UV @ 260 nm; Sample: hydrolyzed -cyclodextrin
-20
20
60
100
10
15
20
25
30
35
mV
Elution Time (minutes)
Columns:
TSKgel G3000PW
XL
-CP, 7µm (7.8m ID x 30cm),
TSKgel G500 PW
XL
-CP, 10µm (7.8mm ID x 30cm),
TSKg l G6 00PW
XL
-CP, 13µm (7.8mm ID x 30cm)
Eluent:
0.1mol/L NaNO
3
Flow Rate:
1mL/min
Detection:
RI
Temperature:
25°C
Sample Load:
3g/L, 100µL
PAA (MW:438kDa)
PAA (MW:235kDa)
PEI (MW:266kDa)
P(DADMACI) (MW:204kDa)
PAS (MW:7800Da)
PAS (MW:287kDa)
Cationic Dextran (MW:11kDa)
Chitosan (MW:13.4kDa)
figure 18
Double stranded DNA calibration curve for TSKgel G-DNA-PW column
figure 19
Oigosaccharides calibration curve for TSKgel G-Oligo-PW column
Elution volume (mL)
Sample:
Mobile phase:
Flow Rate:
Detection:
TSKgel G-Oligo-PW, t
hydr
distil
1.0m
UV
18
16
14
Molecular weight (Da)
200
400
1000
2000
600
800
