sec
SEC
29
Calibration curves for TSKgel ALPHA AND SuperAW Gel Filtration columns
The best results are obtained when selecting a column with the sample’s molecular weight in the linear portion of the calibration curve.
Column:
Eluent:
Flow Rate:
Temperature:
Detection:
TSK-GEL Alpha Series, 7.8 mm ID x 30 cm L
A. H
2
O; B. 10 mmol/L LiBr in Methanol; C. 10 mmol/L LiBr in DMF
1.0 mL/min
A. 25
°
C; B. 25
°
C; C. 40
°
C
RI
2
4
6
8
10
12
14
10
2
10
3
10
4
10
5
10
6
10
7
10
8
Elution Volume (mL)
Molecular Weight (Da)
Polyethylene oxide (PEO), polyethylene glycol (PEG) and polystyrene (PS) calibration curves for TSK-GEL Alpha columns
PS
C.
2
4
6
8
10
12 14
10
2
10
3
10
4
10
5
10
6
10
7
Elution Volume (mL)
Molecular Weight (Da)
PEG
B.
4
6
8
10
12
10
2
10
3
10
4
10
5
10
6
Elution Volume (mL)
Molecular Weight (Da)
PEO
A.
1
2 3 4
5
6
1. Alpha-2500
2. Alpha-3000
3. Alpha-4000
4. Alpha-5000
5. Alpha-6000
6. Alpha-M
1. Alpha-2500
2. Alpha-3000
3. Alpha-4000
4. Alpha-5000
5. Alpha-6000
6. Alpha-M
1. Alpha-2500
2. Alpha-3000
3. Alpha-4000
4. Alpha-5000
5. Alpha-6000
6. Alpha-M
1 23 4
5 6
1
2
3
4 6 5
1
23 4
5
6
1
2
3 4
5
6
1
2
3
4
5
6
Calibration curves for TSK-GEL SuperAW Series in different solvents with different polarity
1
2
3
4
5
6
1 1.5 2 2.5 3 3.5 4
Elution volume (mL)
MW
10
7
10
6
10
5
10
4
10
3
10
2
10
1
2
3
4
5
6 SuperAWM-H
SuperAW6000
SuperAW5000
SuperAW4000
SuperAW3000
SuperAW2500
1
2
3
4
5
6 SuperAWM-H
SuperAW6000
SuperAW5000
SuperAW4000
SuperAW3000
SuperAW2500
1 1.5 2 2.5 3 3.5 4
Elution volume (mL)
MW
10
7
10
6
10
5
10
4
10
3
10
2
10
Column:
TSK-GEL SuperAW Series (6.0 mm ID x 15 cm L)
Eluent:
A. Water; B. MeOH containing 10 mmol/L LiBr; C. DMF containing 10 mmol/L LiBr
Flow rate:
0.6 mL/min
Temperature: 25
°
C
Detection:
Refractive index detector
Samples:
Standard polyethylene oxide, polyethylene glycol, ethylene glycol
1
2
3
4
5
6
1
2
3
4
5
6 SuperAWM-H
SuperAW6000
SuperAW5000
SuperAW4000
SuperAW3000
SuperAW2500
1 1.5 2 2.5 3 3.5 4
Elution volume (mL)
MW
10
7
10
6
10
5
10
4
10
3
10
2
10
1
2
3
4
6 5
A.
B.
C.
Polyethylene oxide (PEO), polyethylene glycol (PEG) and polystyrene (PS) calibration curves for TSKgel Alpha columns
Column: TSKgel Alpha Series, 7.8 mm ID x 30 cm L; Eluent: A. H
2
O; B. 10 mmol/L LiBr in Methanol; C. 10 mmol/L LiBr in DMF; Flow rate: 1.0 mL/min;
Temperature: A. 25°C; B. 25°C; C. 40°C; Detection: RI
alibration curves for TSKgel SuperAW series in diff re t solvents with different polarity
Column: TSKgel SuperAW Series (6.0 mm ID x 15 c L)
Eluent: A. Water; B. MeOH containing 10 mmol/L LiBr; C. DMF containing 10 mmol/L LiBr
Flow rate: 0.6 mL/min; Temperature: 25°C; Detection: Refractive index detector
Samples: Standard polyethylene oxide, polyethylene glycol, ethylene glycol
