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TSKgel SW, SW
XL
and SuperSW Gel Filtration columns
HIGHLIGHTS
Dedicated columns for the analysis of monoclonals available
TSKgel SW-type
columns are all based on spherical silica particles
with very high internal pore volumes.
Silica particles in SW-type columns are chemically bonded
with polar diol groups.
SW-type columns feature low residual adsorption, which is essential
for gel filtration analysis.
Various pore sizes ranges available.
Stainless steel, glass and PEEK column hardware available.
Tosoh recently added three TSKgel SW mAb columns to the renowned
line of TSKgel SW series SEC columns. The TSKgel SW mAb columns
meet the growing demand for the higher resolution and high throughput
separation of monoclonal antibody (mAb) monomer and dimer/fragment,
as well as higher resolution of mAb aggregates. While mAbs can be
analyzed using many different modes of HPLC, size exclusion is best for
determination of aggregate and fragment content.
TSKgel SW series columns contain a large pore volume per unit
column volume, which results in either higher MW selectivity or better
resolution when analyzing proteins. They are based on highly porous
silica particles, the surface of which has been shielded from interacting
with proteins by derivatization with ligands containing diol functional
groups. TSKgel SW series columns stand out from other silica- or
polymer-based high performance size exclusion columns by virtue of
their large pore volumes and low residual adsorption.
TSKgel SW mAb, SW, SuperSW and Ultra SW columns are stable
from pH 2.5 to 7.5 and can be used in 100% aqueous conditions. The
different pore sizes of the TSKgel SW series columns result in different
exclusion limits for globular proteins, polyethylene oxides and dextrans,
as summarized in
Table I
. Furthermore, different particle sizes, column
dimensions and housing materials are available for each of the TSKgel
SW series columns. When the protein analysis needs to be performed
in a metal free environment, the BioAssistSW series offers TSKgel
SW packings in PEEK housings, featuring the same performance as
stainless steel columns.
Recommendations for TSKgel SW series selection
Samples of known molecular weight
Calibration curves for each TSKgel SW series column are provided
in this catalog. Each curve represents a series of various standards
(protein, PEO, or globular proteins, for example) with known molar
masses. The molar mass range of the compound to be analyzed should
be within the linear range of the calibration curve and similar to the
chemical composition and architecture of the calibration standards.
Samples of unknown molecular weight
TSKgel G3000SW
XL
is the ideal scouting column. If the protein of interest
elutes near the exclusion volume, then G4000SW
XL
is the logical next
step. conversely, if the protein of interest elutes near the end of the
chromatogram, try the G2000SW
XL
.
Molecular weight of sample (Da)
TSKgel packing
Particle
Pore
Globular
Dextrans
Polyethylene
size (µm)
size (nm)
proteins
glycols and oxides
SuperSW2000
4
12.5
5 x 10
3
– 1.5 x 10
5
1 x 10
3
–3 x 10
4
5 x 10
2
–15 x 10
3
G2000SW
XL
/BioAssist G2SW
XL
5
12.5
5 x 10
3
– 1.5 x 10
5
1 x 10
3
–3 x 10
4
5 x 10
2
–15 x 10
3
QC-PAK TSK 200
5
12.5
5 x 10
3
– 1.5 x 10
5
1 x 10
3
–3 x 10
4
5 x 10
2
–15 x 10
3
G2000SW
10, 13, 20
12.5
5 x 10
3
– 1.5 x 10
5
1 x 10
3
–3 x 10
4
5 x 10
2
–15 x 10
3
SuperSW3000
4
25
1 x 10
4
– 5 x 10
5
2 x 10
3
–7 x 10
4
1 x 10
3
–3.5 x 10
4
SuperSW mAb
4
25
1 x 10
4
– 5 x 10
5
G3000SW
XL
/BioAssist G3SW
XL
5
25
1 x 10
4
– 5 x 10
5
2 x 10
3
–7 x 10
4
1 x 10
3
–3.5 x 10
4
QC-PAK TSK 300
5
25
1 x 10
4
– 5 x 10
5
2 x 10
3
–7 x 10
4
1 x 10
3
–3.5 x 10
4
G3000SW
10, 13, 20
25
1 x 10
4
– 5 x 10
5
2 x 10
3
–7 x 10
4
1 x 10
3
–3.5 x 10
4
UltraSW Aggregate
3
30
1 x 10
4
– 2 x 10
6
G4000SW
XL
/BioAssist G4SW
XL
8
45
2 x 10
4
– 7 x 10
6
4 x 10
3
–5 x 10
5
2 x 10
3
–2.5 x 10
5
G4000SW
13, 17
45
2 x 10
4
– 7 x 10
6
4 x 10
3
–5 x 10
5
2 x 10
3
–2.5 x 10
5
Data generated using the following conditions:
Columns: Two 4 µm, 4.6 mm ID x 30 cm L TSKgel SuperSW columns in series; two 5 µm, 7.8 mm ID x 30 cm L TSKgel SW
XL
columns in series; two 10 µm,
7.5 mm ID x 60 cm L TSKgel SW columns in series
Elution:
Globular proteins: 0.3 mol/L NaCl in 0.1 mol/L (0.05 mol/L for SW
XL
columns) phosphate buffer, pH 7.0
Dextrans and polyethylene glycols and oxides (PEOs): distilled water
table I
Properties and separation ranges for TSKgel SW-type packings
