19
Iec
However, there is a point of diminishing return. Because
the absolute surface area decreases as the pores become
larger, the insulin capacity decreases accordingly. This
effect is similarly demonstrated by the lysozyme static
binding capacity range specifications of TOYOPEARL
SP-550C and TOYOPEARL SP-650C which are 80-120 g/L
and 35-55 g/L, respectively (data not shown).
For more information on pore size and particle size combi-
nations not contained in our commercial products, please
see the Custom Resin section of this catalog.
Ion exchange
chromatography
(µm)
(eq/L)
(g/L-gel)
(%)
TOYOPEARL GigaCap Q-650S
50-100
0.17
172
97
TOYOPEARL Q-600C AR
50-150
0.18
100
98
TOYOPEARL SuperQ-650M
40-90
0.24
145
98
TOYOPEARL DEAE-650M
40-90
0.11
25
97
TOYOPEARL QAE-550C
50-150
0.36
30
96
Recovery
ANION EXCHANGE RESIN
Particle
size
IEC
DBC (BSA)
TOYOPEARL GigaCap Q-650M
50-100
0.17
172
97
(µm)
(eq/L)
(g/L-gel)
(%)
TOYOPEARL GigaCap S-650M
50-100
0.16
145
98
TOYOPEARL GigaCap CM-650M
50-100
0.24
100
98
TOYOPEARL SP-650C
50-150
0.12
12
98
TOYOPEARL CM-650C
50-150
0.09
35 (lysozyme)
TOYOPEARL SP-550C
50-150
0.13
14
98
Recovery
CATION EXCHANGE RESIN
Particle
size
HW-65
HW-65
HW-65
HW-60
HW-55
Base
bead
HW-65
HW-65
HW-65
HW-65
HW-55
Base
bead
(nm)
100
100
100
75
50
Pore
size
(nm)
100
100
100
100
50
Pore
size
IEC
DBC (hIgG)
TOYOPEARL GigaCap S-650S
50-100
0.16
145
98
HW-65
100
HW-65
100
Base bead
TOYOPEARL HW-65
TSKgel G5000PW
TOYOPEARL HW-60
TOYOPEARL HW-55
TSKgel G3000PW
Pore Diameter
100 nm
75 nm
50 nm
TOYOPEARL GigaCap S-650 TOYOPEARL Q-600C AR TOYOPEARL SP-550
TOYOPEARL GigaCap CM-650
TOYOPEARL MegaCap II SP-550
TOYOPEARL GigaCap Q-650
TOYOPEARL GigaCap DEAE
TOYOPEARL QAE-550
TOYOPEARL SuperQ-650
TOYOPEARL DEAE-650
TOYOPEARL SP-650
TOYOPEARL CM-650
TSKgel SP-5PW
TSKgel SuperQ-5PW
TSKgel DEAE-5PW
TSKgel SP3-PW
25 nm
TABLE I
(µm)
(eq/L)
(g/L-gel)
(%)
TOYOPEARL GigaCap Q-650S
50-100
0.17
172
97
TOYOPEARL Q-600C AR
50-150
0.18
100
98
TOYOPEARL SuperQ-650M
40-90
0.24
145
98
TOYOPEARL DEAE-650M
40-90
0.11
25
97
TOYOPEARL QAE-550C
50-150
0.36
30
96
Recovery
ANION EXCHANGE RESIN
Particle
size
IEC
DBC (BSA)
TOYOPEARL GigaCap Q-650M
50-100
0.17
172
97
(µm)
(eq/L)
(g/L-gel)
(%)
TOYOPEARL GigaCap S-650M
50-100
0.16
145
98
TOYOPEARL GigaCap CM-650M
50-100
0.24
100
98
TOYOPEARL SP-650C
50-150
0.12
12
98
TOYOPEARL CM-650C
50-150
0.09
35 (lysozyme)
TOYOPEARL SP-550C
50-150
0.13
14
98
Recovery
CATION EXCHANGE RESIN
Particle
size
H -65
H -65
HW-65
HW-60
HW-55
Base
bead
HW-65
HW-65
HW-65
HW-65
HW-55
Base
bead
(nm)
100
100
100
75
50
Pore
size
(nm)
100
100
100
100
50
Pore
size
IEC
DBC (hIgG)
TOYOPEARL GigaCap S-650S
50-100
0.16
145
98
H -65
100
HW-65
100
Base bead
TOYOPEARL HW-65
TSKgel G5000PW
TOYOPEARL HW-60
TOYOPEARL HW-55
TSKgel G3000PW
Pore Diameter
100 nm
75 nm
50 nm
TOYOPEARL GigaCap S-650 TOYOPEARL Q-600C AR TOYOPEARL SP-550
TOYOPEARL GigaCap CM-650
TOYOPEARL MegaCap II SP-550
TOYOPEARL GigaCap Q-650
TOYOPEARL GigaCap DEAE
TOYOPEARL QAE-550
TOYOPEARL SuperQ-650
TOYOPEARL DEAE-650
TOYOPEARL SP-650
TOYOPEARL CM-650
TSKgel SP-5PW
TSKgel SuperQ-5PW
TSKgel DEAE-5PW
TSKgel SP3-PW
25 nm
TABLE II
figure 2
Optimization of insulin binding capacity as
a function of pore size of experimental
TSK-GEL SP-type resins
0
50
100
150
200
10
3
10
4
10
5
10
6
Insulin binding capacity
(g/L-gel)
Exclusion limit
Increasing target
accessibility
Decreasing pore
surface area
optimization of insulin binding capacity as a function
of pore size of experimental tskgel sp-type resins
