11
SEC
size exclusion
chromatography
Properties and molecular weight separation ranges for TOYOPEARL HW-type resins
(HW = Hydrophilic, Water-compatible polymeric base resins)
Molecular weight of sample (Da)
TOYOPEARL PARTICLE
PORE
POLYETHYLENE
DEXTRANS
GLOBULAR
RESIN
SIZE (µm)
SIZE (nm)
GLYCOLS AND OXIDES
PROTEINS
HW-40S
20 - 40
5
100 - 3,000
100 - 7,000
100 - 10,000
HW-40F
30 - 60
HW-40C
50 - 100
HW-50S
20 - 40
12,5
100 - 18,000
500 - 20,000
500 - 80,000
HW-50F
30 - 60
HW-55S
20 - 40
50
100 - 150,000
1,000 - 200,000
1,000 - 700,000
HW-55F
30 - 60
HW-65S
20 - 40
100
500 - 1,000,000
10,000 - 1,000,000
40,000 - 5,000,000
table I
HW-40
HW-50
HW-55 *
HW-65 **
HW-75
10
2
10
3
10
4
10
5
10
6
10
7
HW-40
HW-50
HW-55
HW-65
HW-75
HW-40
HW-50
HW-55
HW-65
HW-75
Leucine
Vitamin B12
ACTH
Insulin
Cytochrome C
Ribonuclease A
α
-Chymotrypsinogen
Ovalbumin
Albumin
Human
γ
-Globulin
Aldolase
Catalase
Urease
Thyroglobulin
Blue Dextran
Poliovirus RNA
Hemocyanin
table II
A) GLOBULAR PROTEINS
* HW-55 is base material for
some IEC and HIC products
** HW-65 is base material
for most IEC, HIC and AFC
products
Calibration
molecules
b) DextranS
c) Polyethylene
glycols
