AFC
AFC
97
ORDERING INFORMATION
Part #
Description
ID Length
Particle
Number
Flow rate (mL/min)
Maximum
(mm)
(cm)
size (µm)
theoretical
range
pressure drop
plates
(MPa)
Glass columns
0014449 Boronate-5PW Glass, 100 nm 5.0
5.0
10
≥
500
0.5 - 1.0
2.0
0014440 Chelate-5PW Glass, 100 nm 5.0
5.0
10
≥
500
0.5 - 0.8
2.0
TSKgel Stainless Steel Columns
0013066 Boronate-5PW, 100 nm
7.5
7.5
10
≥
1,300
0.5 - 1.0
1.0
0008645 Chelate-5PW, 100 nm
7.5
7.5
10
≥
1,300
0.5 - 1.0
1.0
0014455 Tresyl-5PW, 100 nm
6.0
4.0
10
0.2 - 0.5
1.0
0014456 Tresyl-5PW, 100 nm
7.5
7.5
10
0.5 - 1.0
1.0
TSKgel PEEK columns
0020022 BioAssist Chelate, 100 nm 7.8
5.0
10
≥
800
0.5 - 1.0
1.0
Guard column products
0014451 Boronate-5PW Glass Guardgel Kit
20
For P/N 0014449
0013125 Boronate-5PW Guardgel Kit
For P/N 0013066
0008647 Chelate-5PW Guardgel Kit
For P/N 0008645
Bulk packing
0016208 Tresyl-5PW, 2 g dry gel*
10
* 1 g is approximately 3.5 mL
Principal applications for TSKgel Tresyl-5PW include the selective
purification of antigens after coupling the appropriate antibody to the
solid support. The antibody coupling yield at pH >7.5 is more than 90 %,
with the maximum binding occurring at pH 7.5. Antigen adsorption to the
antibody ligand is most effective when the antibody concentration is
< 2-3 mg/mL of affinity resin. To increase binding capacity, more
antibody should be added to the coupling reaction.
However, higher concentrations of antibody can result in steric
hindrance, thus lowering the binding capacity of the column.
As a general rule, the time required for antibody attachment to
the TSKgel Tresyl-5PW column is directly proportional to the
antibody concentration. Small amounts of antibody require about
2 hours to complete the cross-linking reaction, whereas it may
take 6-7 hours to fully attach an antibody at the concentration of
10 mg/mL-resin.
Examples of the wide range of applications using TSKgel Tresyl-5PW
include the binding of such ligands as concanavalin A (a lipoprotein
lectin that binds to glycoproteins), numerous antibodies and enzymes.
The chromatogram in
Figure 4
shows the purification of peroxidase
by the concanvalin A ligand coupled to the TSKgel Tresyl-5PW affinity
support resin.
