IEC
48
TOSOH BIOSCIENCE
TSKgel ANION EXCHANGE COLUMNS
HIGHLIGHTS
TSKgel Q- and DNA-STAT columns provide high efficiency
separations at short analysis time.
TSKgel DNA-NPR columns are ideal for PCR fragment analysis.
TSKgel SuperQ-5PW columns have higher capacity than TSKgel
DEAE-5PW due to novel bonding chemistry, effective pore size is
smaller for SuperQ-5PW.
Pore structure and bonding chemistry of TSKgel BioAssist Q
columns provide high capacity for small to very large MW proteins
and nucleic acids.
BioAssist columns are packed in 4.6 mm ID or 10 mm ID PEEK
hardware. Other columns are available in glass and stainless steel
for analytical, semi-preparative and preparative applications.
Binding capacity for small to medium size proteins on TSKgel DEAE-
3SW is roughly double that of the DEAE-5PW due to the smaller pore
size and larger surface area.
Specialty columns for analysis of mono- and disaccharides and
sugar alcohols are also available.
Non-porous TSKgel STAT Anion Exchange Columns
STAT columns are available in various column formats and particle
sizes to perfectly match specific application needs. For fast and ultra-
fast analysis anion and cation exchange columns in 3 mm ID and 3.5 cm
length are packed with 10 μm particles. They are ideally suited for rapid
candidate screening or process monitoring. 4.6 mm ID and 10 cm length
columns packed with 7 μm particles are designed for high resolution
IEC separation for example for the separation of nucleic acids, mAb
variants, PEGylated protein or protein aggregates.
The DNA STAT column (4.6 mm ID x 10 cm L) packed with 5 μm Q-type
anion exchange resin is ideally suited for the analysis of nucleic acids.
The basic properties of TSKgel STAT anion exchange columns are
summerized in
TABLE I
.
TABLE I
Basic properties of TSKgel STAT Anion Exchange columns
Applications of TSKgel ANION exchange columns
Property
TSKgel Q-STAT
TSKgel DNA-STAT
Base material
Cross-linked hydrophilic polymer (mono-disperse particles)
Pore size
Non-porous
Functional group
Quaternary ammonium (same chemistry)
Particle size
7 µm
10 µm
5 µm
Column size
4.6 mm ID x 10 cm L
3 mm ID x 3.5 cm L
4.6 mm ID x 10 cm L
Application
High resolution
protein separation
High resolution
protein separation
High resolution
DNA separations
