Basic HTML Version
SEC
42
The best results are obtained when selecting a column with the sample’s
molecular weight in the linear portion of the calibration curve.
Column: TSKgel PW columns: A. G2000PW, B. G2500PW, C. G3000PW,
D. G4000PW, E. G5000PW, F. G6000PW, G. GMPW, all 7.5mm ID x 60 cm L
TSKgel PW
XL
columns: H. G2500PW
XL
, J. G3000PW
XL
, K. G4000PW
XL
,
L. G5000PW
XL
, M. G6000PW
XL
, N. GMPW
XL
, all 7.8 mm ID x 30 cm L; Elution:
distilled water; Flow Rate: 1.0 m L/min; Detection: RI
Column: 1. TSKgel G3000PW
XL
, 2. G4000PW
XL
, 3. G5000PW
XL
, 4. G6000PW
XL
,
5. GMPW
XL
; Sample: a. thyroglobulin (660,000 Da), b.
g
-globulin (150,000 Da),
c. albumin (67,000 Da), d. ovalbumin (43,000 Da), e.
b
-lactoglobulin (36,000
Da), f. myoglobin (16,900 Da), g. cytochrome C (12,400 Da); Elution: 0.2 mol/L
phosphate buffer (pH 6.8); Flow Rate: 1.0 mL/min; Detection: UV @ 280 nm
Columns: TSKgel G3000PW
XL
-CP, 7 μm, 7.8 mm ID x 30 cm L, TSKgel
G5000PW
XL
-CP, 10 μm, 7.8 mm ID x 30 cm L, TSKgel G6000PW
XL
-CP, 13 μm,
7.8 mm ID x 30 cm L
Mobile phase: 0.1 mol/L NaNO
3
; Flow Rate: 1 mL/min; Detection: RI;
Temperature: 25°C; Samples: polyethylene oxides (PEO) standards,
polyethylene glycols (PEG) standards
Columns:TSKgelSuperOligoPW,SuperMultiporePW-N,SuperMultiporePW-M,
SuperMultiporePW-H (each 6.0 mm ID x 15 cm L);
Mobile phase: H
2
O; Flow rate: 0.60 mL/min; Detection: RI; Temperature: 25°C;
Samples: polyethylene oxides (PEO) standards, polyethylene glycols (PEG)
standards, ethylene glycol (EG) standards
Polyethylene glycol and oxide calibration curves
on TSK-GEL PW and TSK-GEL PW
XL
columns
Elution volume (mL)
Colu n:
Elution:
Flow Rate:
Detection:
TSK-GEL PW columns: A. G2000PW, B. G2500PW, C. G3000PW
D. G4000PW, E. G5000PW, F. 6000 , G. GMPW, all 7.5m ID x 60 cm
TSK-GEL PW
XL
columns: H. 25
XL
J 3
XL
K. G4000PW
XL
,
L. G5000PW
XL
, M. G6000PW
XL
, N. GMPW
XL
, all 7.8 m ID x 30 cm
distilled water
1.0 m L/min
RI
5
TSK-GEL PW Columns
TSK-GEL PW
XL
Columns
Molecular weight (Da)
10
15
10
20
15
10
2
10
3
10
4
10
5
10
6
H
J
N
K
L
M
10
2
10
3
10
4
10
5
10
6
G
E F
C
D
A B
Elution volume (mL)
Column:
1. G3000PW
XL
, 2. G4000PW
XL
, 3. G5000PW
XL
,
4. G6000PW
XL
, 5. GMPW
XL
Sample:
a. thyroglobulin (660,000 Da), b.
γ
-globulin (150,000 Da),
c. albumin (67,000 Da), d. ovalbumin (43,000 Da),
e.
β
-lactoglobulin (36, 00 Da), f. myoglobin (16,900 Da),
g. cytochrome C (12,400 Da)
Elution:
0.2 mol/L phosphate buffer (pH 6.8)
Flow Rate: 1.0 mL/min
Detection: UV @ 280 nm
Molecular weight (Da)
10
4
10
3
10
5
10
6
10
7
5
1
3
2
4
a
b
c
d e
f
g
Protein calibration curves on TSK-GEL PW
XL
columns
4
6
8
10
12
Elution volume (mL)
figure 7
Polyethylene glycol and oxide calibration curves on TSKgel PW and
TSKgel PW
XL
columns
figure 8
Protein calibration curves on TSKgel PW
XL
columns
figure 9
Polyethylene Glycol and Oxide Calibration Curves for TSKgel PW
XL
-CP
Columns
TSKgel G3
TSKgel G5
TSKgel G6
Mobile pha
Flow Rate:
Detection:
Temp:
Samples:
10
10
2
10
3
10
4
10
5
10
6
10
7
3
5
7
9
11
Elution time (minutes)
Log Molecular Weight (PEO)
TSKgel G3000PW
XL
-CP
TSKgel G5000PW
XL
-CP
TSKgel G6000PW
XL
-CP
figure 10
Polyethylene Glycol, Oxide and Ethylene Glycol Calibration Curves for
TSKgel SuperMultiporePW and SuperOligoPW
10
10
2
10
3
10
4
10
5
10
6
10
7
1.50
2.50
3.50
4.50
5.50
Elution time (minutes)
Log Molecular Weight
TSKgel SuperOligoPW
TSKgel SuperMultiporePW-N
TSKgel SuperMultiporePW-M
TSKgel SuperMultiporePW-H
TSKgel Su
TSKgel Su
TSKgel Su
TSKgel Su
Mobile pha
Flow rate:
Detection:
Temperatu
Samples:
Calibration curves for TSKgel PW / supermultipore PWGel Filtration columns