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TSKgel PW and TSKgel PW
XL
columns -
Gel Filtration Chromatography of water soluble polymers
HIGHLIGHTS
Hydrophilic, rigid, spherical, porous methacrylate beads
pH range of 2 to 12, with up to 50% organic solvent
Temperatures up to 80°C (50°C for TSKgel G-DNA-PW)
Wide separation range up to 8 x 10
6
Da for linear polymers
Linear SEC column line incorporating proprietary multi-pore
technology
Specialty columns for low salt separation of cationic polymers
Polymeric TSKgel PW and high resolution TSKgel PW
XL
columns are
designed for SEC of water soluble organic polymers, polysaccharides,
DNA, and RNA. They are based on a hydrophilic polymethacrylate
matrix. For analytical purposes the TSKgel PW
XL
columns are preferred,
whereas for preparative work the 60 cm TSKgel PW columns are
recommended because of their higher loading capacity. For the
analysis of proteins and peptides we recommend to use silica based
SW type columns.
A number of specialty columns include columns for samples with a
broad molecular weight range, oligosaccharides, DNA and RNA. A
large pore G6000PW phase is available in PEEK column hardware
(TSKgel BioAssist G6PW) for ultra-low sample adsorption during
virus analysis. TSKgel PW
XL
-CP columns are especially suited for the
separation of cationic polymers.
The latest addition to the TSKgel PW family are high resolution semi
micro columns for oligomer analysis (TSKgel SuperOligoPW) and for
analysis of MW distribution of by linear SEC (TSKgel SuperMultipore
PW).
TSKgel Column
Particle size (μm)
Pore size (Å)
MW range
(PEG/PEO)
Dextrans*
G1000PW
12
< 100
< 1 x 10
3
G2000PW
12
125
< 2 x 10
3
G2500PW
12, 17
< 200
< 3 x 10
3
< 3 x 10
3
G3000PW
12, 17
200
< 5 x 10
4
G4000PW
17
500
< 3 x 10
5
G5000PW
17
1,000
< 1 x 10
6
G6000PW/ BioAssist G6PW
17
> 1,000
< 8 x 10
6
GMPW
17
< 100 - 1,000
5 x 10
2
- 8 x 10
6
G2500PW
XL
7
< 200
< 3 x 10
3
G3000PW
XL
7
200
< 5 x 10
4
< 6 x 10
4
G4000PW
XL
10
< 500
< 3 x 10
5
1 x 10
3
- 7 x 10
5
G5000PW
XL
10
1000
< 1 x 10
6
5 x 10
4
- 2.5 x 10
6
G6000PW
XL
13
> 100
< 8 x 10
6
5 x 10
5
- 5 x 10
7
G-DNA-PW
10
> 1,000
< 8 x 10
6
< 5 x 10
7
GMPW
XL
13
100 - 1,000
5 x 10
2
- 8 x 10
6
< 5 x 10
7
G-Oligo-PW
7
125
< 5 x 10
3
SuperMultiporePW-N
4
n/a
3 x 10
2
- 5 x 10
4
SuperMultiporePW-M
5
n/a
5 x 10
2
- 1 x 10
6
SuperMultiporePW-H
8 (6-10)
n/a
1 x 10
3
- 1 x 10
7
SuperOligoPW
3
n/a
1 x 10
2
- 3 x 10
3
G3000PW
XL
-CP
7
200
< 9 x 10
4
G5000PW
XL
-CP
10
1,000
< 1 x 10
6
G6000PW
XL
-CP
13
> 1,000
< 2 x 10
7
Column: TSKgel PW columns, 7.5 mm ID x 60 cm L; TSKgel PW
XL
, TSKgel PW
XL
-CP, G-Oligo-PW & G-DNA-PW, 7.8 mm ID x 30 cm L
Elution: Polyethylene glycols and oxides: distilled water; dextrans: 0.2 mol/L phosphate buffer, pH 6.8
Flow rate: 1.0 mL/min, except for TSKgel SuperMultiporePW and TSKgel SuperOligoPW columns: 0.6 mL/min
Note: *Maximum separation range determined from estimated exclusion limits
table 2
Properties and Separation Ranges for TSKgel PW-Type Packings