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SEC
36
Calibration curves for TSKgel SW-type Gel Filtration columns
Polyethylene oxide, dextran and protein calibration curves for TSK-GEL SW columns
TSKgel G2000SW
TSKgel G4000SW
TSKgel G3000SW
Column:
Sample:
Elution:
Flow Rate:
Detection:
10
6
Molecular weight (Da)
10
5
Elution volume (mL)
Elution volume (mL)
TSK-GEL SW, two 7.5 mm ID x 60 cm L columns in series
proteins, polyethylene oxides, dextrans
dextrans and polyethylene oxides: distilled water; proteins: 0.3 mol/L NaCl in 0.1 mol/L phosphate buffer, pH 7.0
1.0 mL/min
UV @ 220 nm and RI
40
20
30
40
20
30
10
6
10
6
10
5
10
5
10
3
10
3
10
4
10
4
10
4
10
3
40
20
30
Elution volume (mL)
Protein calibration curves for TSK-GEL SW
XL
columns
TSKgel G2000SW
XL
TSKgel G3000SW
XL
TSKgel G4000SW
XL
Elution volume (mL)
6
10
12
Column:
Sample:
Elution:
Detection:
TSK-GEL SW
XL
columns, 5 or 8
µ
m ,7.8 mm ID x 30 cm L
1. thyroglobulin (660,000 Da ); 2. IgG (160,000 Da );
3. BSA (67,000 Da); 4. ovalbumin (43,000 Da);
5. peroxidase (40,200 Da); 6.
β
-lactoglobulin (18,400 Da);
7. myoglobin (16,900 D a) 8. ribonuclease A (12,600 Da);
;
9. cytochrome C (12,400 Da); 10. glycine tetramer (246 Da)
0.3 mol/L NaCl in 0.1 mol/L sodium phosphate buffer, pH 7.0
UV @ 220 nm
10
2
10
3
10
4
10
5
10
6
1
3
5
7 8
6
4
9
2
10
Molecular weight (Da)
8
5 6 7 8 9 10 11 12
Minutes
Calibration curves for TSK-GEL SuperSW and SW
XL
Sample:
Elution:
Flow Rate:
Temperature:
Detection:
proteins: 1. thyroglobulin (660,000 Da);
2.
γ
-globulin (150,000 Da); 3. BSA (67,000 Da);
4.
β
-lactoglobulin (18,400 Da); 5. lysozyme (14,500 Da);
6. cytochrome C (12,400 Da); 7. triglycine (189 Da)
0.15 mol/L phosphate buffer (pH 6.8)
0.35 mL/min for SuperSW; 1.0 mL/min for SW
XL
25
°
C
UV @ 280 nm (220 nm for triglycine)
10
2
10
3
10
4
10
5
10
6
Molecular weight (Da)
SuperSW2000
G2000SW
XL
SuperSW3000
1
2
3
4
5
6
7
G3000SW
XL
The best results are obtained when selecting a column with the sample’s molecular weight in the linear portion of the calibration curve.
Calibration curves for tskgel super sw and SW
XL
Protein calibration curves for tskgel SW
XL
columns
Polyethylene oxide, dextran and protein calibration curves for tskgel sw columns