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TSKgel SW, SW
XL
and SuperSW Gel Filtration columns
HIGHLIGHTS
TSKgel SW-type
columns are all based on spherical silica particles
with very high internal pore volumes.
Silica particles in SW-type columns are chemically bonded
with polar diol groups.
SW-type columns feature low residual adsorption, which is essential
for gel filtration analysis.
Three pore sizes ranges (125 Å, 250 Å and 450 Å) available.
Stainless steel, glass and PEEK column hardware available.
TSKgel SW series columns (SW, SW
XL
and Super SW) contain a large
pore volume per unit column volume, which results in either higher MW
selectivity or better resolution when analyzing proteins. They are based
on highly porous silica particles, the surface of which has been shielded
from interacting with proteins by derivatization with ligands containing
diol functional groups. TSKgel SW series columns stand out from other
silica- or polymer-based high performance size exclusion columns by
virtue of their large pore volumes and low residual adsorption.
SW and SW
XL
columns are available in three pore size ranges with
nominal pore sizes of 125 Å, 250 Å and 450 Å. SuperSW and QC-PAK
column lines are available in 125 Å and 250 Å. SW columns are packed
with 10 micron (G2000SW and G3000SW) or 13 micron (G4000SW)
particles. SW
XL
columns contain 5 micron (G2000SW
XL
and G3000SW
XL
)
or 8 micron (G4000SW
XL
) particles. SuperSW columns contain 4 micron
particles.
Recommendations for TSKgel SW series selection Samples
of unknown molecular weight
TSKgel G3000SW
XL
is the ideal scouting column. If the protein of interest
elutes near the exclusion volume, then G4000SW
XL
is the logical next
step. Conversely, if the protein of interest elutes near the end of the
chromatogram, try the G2000SW
XL
.
Proteins (general)
Choose one of the TSKgel SW
XL
columns using the calibration curves
on page 34 to select the appropriate pore size based on knowledge or
estimate of protein size.
Monoclonal antibodies
TSKgel G3000SW
XL
is commonly used for quality control. TSKgel
SuperSW3000 is utilized when sample is limited or at very low
concentration.
Peptides
TSKgel G2000SW
XL
is the first selection for the analysis of peptides.
TSKgel SuperSW2000 is utilized when sample is limited or at very low
concentration.
Other
The use of TSKgel SuperSW columns requires optimization of the HPLC
system with respect to extra-column band broadening. Capillary tubing
ID, injection volume, detector cell volume, and detector time constant
all need to be reduced to fully benefit from the high column efficiency
and small peak volumes of the SuperSW columns. Use SW columns
when not sample limited or when larger amounts of sample need to be
isolated.
Molecular weight of sample (Da)
TSKgel packing
Particle
Pore
Globular
Dextrans
Polyethylene
size (µm)
size (Å)
proteins
glycols and oxides
SuperSW2000
4
125
5 x 10
3
– 1.5 x 10
5
1 x 10
3
–3 x 10
4
5 x 10
2
–15 x 10
3
G2000SW
XL
/BioAssist G2SW
XL
5
125
5 x 10
3
– 1.5 x 10
5
1 x 10
3
–3 x 10
4
5 x 10
2
–15 x 10
3
QC-PAK TSK 200
5
125
5 x 10
3
– 1.5 x 10
5
1 x 10
3
–3 x 10
4
5 x 10
2
–15 x 10
3
G2000SW
10, 13, 20
125
5 x 10
3
– 1.5 x 10
5
1 x 10
3
–3 x 10
4
5 x 10
2
–15 x 10
3
SuperSW3000
4
250
1 x 10
4
– 5 x 10
5
2 x 10
3
–7 x 10
4
1 x 10
3
–3.5 x 10
4
G3000SW
XL
/BioAssist G3SW
XL
5
250
1 x 10
4
– 5 x 10
5
2 x 10
3
–7 x 10
4
1 x 10
3
–3.5 x 10
4
QC-PAK TSK 300
5
250
1 x 10
4
– 5 x 10
5
2 x 10
3
–7 x 10
4
1 x 10
3
–3.5 x 10
4
G3000SW
10, 13, 20
250
1 x 10
4
– 5 x 10
5
2 x 10
3
–7 x 10
4
1 x 10
3
–3.5 x 10
4
G4000SW
XL
/BioAssist G4SW
XL
8
450
2 x 10
4
– 7 x 10
6
4 x 10
3
–5 x 10
5
2 x 10
3
–2.5 x 10
5
G4000SW
13, 17
450
2 x 10
4
– 7 x 10
6
4 x 10
3
–5 x 10
5
2 x 10
3
–2.5 x 10
5
Data generated using the following conditions:
Columns: Two 4 µm, 4.6 mm ID x 30cm L TSKgel SuperSW columns in series; two 5 µm, 7.8 mm ID x 30 cm L TSKgel SW
XL
columns in series; two 10 µm,
7.5 mm ID x 60 cm L TSKgel SW columns in series
Elution:
Globular proteins: 0.3 mol/L NaCl in 0.1 mol/L (0.05 mol/L for SW
XL
columns) phosphate buffer, pH 7.0
Dextrans and polyethylene glycols and oxides (PEOs): distilled water
table 1
Properties and Separation Ranges for TSKgel SW-Type Packings