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SEC
32
INTRODUCTION TO TSKgel SIZE EXCLUSION COLUMNS
Gel Filtration Chromatography (GFC)
GFC is popular among biochemists for the isolation of proteins, for the
removal of aggregates, to desalt a protein sample, to separate nucleic
acid fractions, or to characterize water-soluble polymers used in food
products, paints, pharmaceutical preparations, etc. Available TSKgel
products are classified by application area and particle composition.
Each of the types below is described in detail in this chapter.
Application Area:
Proteins and other biopolymers
Base material: silica
• SW
• SW
XL
• SuperSW
These columns are ideal for proteins and nucleic acids using an
aqueous buffer as mobile phase.
Application Area:
Water-soluble polymers
Base material: polymethacrylate
• PW
• SuperMultiporePW
• SuperOligoPW
• PW
XL
• PW
XL
-CP
These columns are ideal for industrial polymers, oligosaccharides,
nucleic acids and small viruses using aqueous buffer or salt solutions
as mobile phase. The new TSKgel SuperMultiporePW semi-micro SEC
columns provide near linear calibration curves and are ideally suited
to analyze the MW distribution of water soluble polymers with a wide
range of molecular weights. The SuperOligoPW semi-micro column
featuring a small particle size has been designed for fast analysis
of oligosaccharides and other oligomers. The PW
XL
-CP columns are
developed to facilitate SEC separation of cationic polymer under low
salt conditions.
Application Area:
Water- and organic-soluble polymers
Base material: highly crosslinked polymethacrylate
• Alpha
• SuperAW
These columns are ideal for industrial polymers soluble in water,
buffers and many organic solvents.
Gel Permeation Chromatography (GPC)
GPC plays an important role in the characterization of organic-soluble
polymers in the chemical and petrochemical industries. TSKgel GPC
columns contain particles prepared from polystyrene crosslinked with
divinylbenzene.
The proprietary multi-pore particle technology applied in some linear
GPC columns ensures a wide pore size distribution in each particle
leading to calibration curves with excellent linearity. Available GPC
columns are grouped according to their relative lack of adsorptive
properties and the speed of analysis.
Each of the types below is described in detail in this chapter.
Application Area:
Organic-soluble polymers
Base material: polystyrene
Ultra-low adsorption columns with limited solvent range
• SuperHZ (high throughput)
• SuperMultiporeHZ
• H
XL
(conventional)
Low adsorption columns with expanded solvent range
• SuperH (high throughput)
• H
HR
(conventional)
FEATURES
BENEFITS
Rigid hydrophilic and hydrophobic packings
Minimal swelling and excellent physical strength
Low adsorption resulting in high mass recovery
Four series of SEC columns with diferent ranges of solvent
compatibility
Suitable for both types of size exclusion, aqueous (GFC) and
non-aqueous (GPC)
Easy scale up
Analytical and preparative pre-packed SEC column