TOSOH
CUSTOMER MAGAZINE
TOYOPEARL AF-rProtein A HC-650F is a new Protein A chromato-
graphy resin designed for the purification of monoclonal antibodies
(mAbs). It is well-suited for high capacity capturing of immunoglobu-
lin out of high titer feedstocks and achieves 30% to 50% greater an-
tibody adsorption than similar products. It exhibits dynamic binding
capacities of greater than 70 g/L at residence times of 5 minutes, and
greater than 50 g/L at 2 minutes with feedstock titers from 1 g/L to
more than 10 g/L.
The recombinant ligand that is linked to the well-proven methacry-
lic polymer backbone of TOYOPEARL media has been engineered to
maintain capacity even after repeated exposure to alkaline solution.
Its multipoint attachment to the TOYOPEARL matrix further enhances
chemical and thermal stability. In practice this pays off for a low level
of Protein A leaching and also for a high resistance to alkaline solu-
tions applied in cleaning-in-place (CIP) procedures.
Protein A resins constitute a substantial cost in state-of-the-art mAb
purification processes. Factors such as operating cycles, capacity, and
mAb titer have an impact on total costs associated with mAb purifica-
tion. The high capacity of the new TOYOPEARL AF-rProtein A HC-650F
and its high alkaline resistance increase product throughput, reduce
operating costs, and increase manufacturing productivity.
MiniChrom for TOYOPEARL and TSKgel are part of the method de-
velopment platform from Tosoh Bioscience. They are designed for
method optimization, parameter screening, and/or small scale purifi-
cation. The columns (5 mL, 8 mm ID x 10 cm L) are made of biocom-
patible polyethylene and polypropylene and are prepacked with a ran-
ge of TOYOPEARL or TSKgel chromatography resins under optimum
compression, ensuring consistent experimental results. MiniChrom
columns can be connected directly to any laboratory liquid chromato-
graphy system via standard HPLC connectors.
The 5 mL MiniChrom columns are the ideal tool to further optimize the
purification method and to confirm the operational window after ha-
ving selected a resin for a certain purification task by resin screening,
e.g. with ToyoScreen RoboColumns on robotic workstations. The co-
lumns are packed by Atoll GmbH and are available with a broad range
of ion exchange, hydrophobic interaction, mixed-mode, gel filtration,
and Protein A affinity media.
WIN A TOYOPEARL AF-rProtein A HC-650F 5 mL MiniChrom COLUMN
A
TOYOPEARL HIGH-CAPACITY PROTEIN A RESIN AND
MiniChrom COLUMNS
03
WHAT’S NEW
RESINS
AFTER A SUCCESSFUL INTRODUCTION OF NEW SEC COLUMNS FOR mAb ANALYSIS LAST SUMMER WE ARE HAPPY TO ANNOUNCE
SOME INSPIRING PRODUCT INTRODUCTIONS FOR ANTIBODY PURIFICATION AND BIOPURIFICATION IN GENERAL. THE FAMILY OF ALKALI
STABLE TOYOPEARL PROTEIN A RESINS WAS EXPANDED BY AN ULTRA-HIGH CAPACITY RESIN. IN ADDITION, THE MOST POPULAR TOYO-
PEARL AND TSKgel RESINS ARE NOW AVAILABLE IN A NEW COLUMN FORMAT, THE 5 mL MiniChrom COLUMNS FOR PROCESS DEVELOP-
MENT, PARAMETER SCREENING AND MICRO SCALE PURIFICATIONS.
0
1
2
3
4
5
6
7
Residence time (min)
30
40
50
60
70
80
DBC for IgG, 10% Breathrough (mg/mL)
Mab Select SuRe
TM
Mab Select SuRe
TM
LX
TOYOPEARL AF-rProtein A HC
COMPARISON OF BINDING CAPACITIES OF THREE ALKALI STABLE
PROTEIN A RESINS
MIXED-MODE SEPARATION OF ANTIBODY AND AGGREGATES ON MiniChrom
TOYOPEARL MX-Trp-650M
100
200
300
volume [mL]
600
400
200
0
UV absorbance @ 280 nm [mAU]
aggregated mAb
mAb
monomer
monomer
aggregates
CIP
CIP
