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16
RPC
Polymer based rp columns TSKgel OCTADECYL-NPR / -2PW / -4PW/ -PHENYL-5PW RP
HIGHLIGHTS
Polymer-based RPC columns are chemically stable at pH 2-12,
allowing operation at basic pH where silica-based columns have
limited chemical stability.
Polymer-based columns can be cleaned and impurities removed by
using either strong acid or base.
Non-porous resins (NPR) or porous resins of various pore sizes
available. Column selection is based on sample MW or application.
2.5 µm particle size TSKgel Octadecyl-NPR resin features fast
kinetics resulting in high column efficiency and quantitative protein
recovery at sub-microgram loads.
TSKgel Octadecyl-2PW with 5 µm particle size and 125 Å pores size.
TSKgel Octadecyl-4PW with 7 µm particle size and 500 Å pores size.
TSKgel Phenyl-5PW with 10 µm particle size and an average pore
size of 1000 Å. In comparison with the Phenyl-5PW packing material
used in HIC, the greater level of hydrophobicity in TSKgel Phenyl-
5PW RP makes this material more suitable for use in RPC.
APPLICATIONS
TSKgel OCTADECYL-NPR
High efficiency purification of proteins and peptides at sub-
microgram loads
Stable to higher pressures than porous particles
Improved recovery at low sample concentration over traditional
porous resins
TSKgel OCTADECYL-2PW
For analyzing small MW pharmaceutical compounds at basic pH
Faster analysis than competitive polymeric RPC columns
TSKgel OCTADECYL-4PW
Recommended for peptides and small proteins
TSKgel PHENYL-5PW RP
Ideal for the separation of proteins, including high MW
Able to handle high loads (high capacity)
ORDERING INFORMATION
Part #
Description
ID Length Particle
Number
Flow rate (mL/min)
Maximum
(mm)
(cm)
size (µm)
theoretical
Range
Max.
pressure
plates
drop (MPa)
TSKgel Stainless Steel Columns
14005 Octadecyl-NPR nonporous
4.6
3.5
2.5
≥
1,000 1.0 - 1.5
1.6
20.0
18754 Octadecyl-2PW, (100 - 200 Å)
2.0
15.0
5
≥
5,000 0.07 - 0.11
0.14
7.0
17500 Octadecyl-2PW, (100 - 200 Å)
4.6
15.0
5
≥
6,000 0.4 - 0.6
1.2
10.0
17501 Octadecyl-2PW, (100 - 200 Å)
6.0
15.0
5
≥
6,000 0.5 - 1.0
1.5
10.0
18755 Octadecyl-4PW, 500 Å
2.0
15.0
7
≥
2,000 0.08 - 0.17
0.22
10.0
13351 Octadecyl-4PW, 500 Å
4.6
15.0
7
≥
2,000 0.5 - 1.0
1.2
12.0
16257 Octadecyl-4PW, 500 Å
21.5
15.0
13
≥
2,000 3.0 - 6.0
8.0
3.5
18756 Phenyl-5PW RP, 1000 Å
2.0
7.5
10
≥
400 0.05 - 0.1
0.12
1.0
08043 Phenyl-5PW RP, 1000 Å
4.6
7.5
10
≥
500 0.5 - 1.0
1.2
3.0
16260 Phenyl-5PW RP, 1000 Å
21.5
15.0
13
≥
1,000 6.0 - 8.0
8.0
3.0
Glass columns
14007 Phenyl-5PW RP Glass, 1000 Å
8.0
7.5
10
≥
700 1.0 - 2.0
2.0
Guard column products
19007 Phenyl-5PW RP Cartridge, pk 3 *
3.2
1.5
10
For P/N 08043
17502 Octadecyl-2PW Guard column
4.6
1.0
5
For P/N 17500
17503 Octadecyl-2PW Guard column
6.0
1.0
5
For P/N 17501
19008 Octadecyl-4PW Cartridge, pk 3
*
3.2
1.5
7
For P/N 13351
19308 Guard cartridge holder
2.0
1.0
For all 2 mm ID cartridges
19018 Guard cartridge holder
3.2
1.5
For 4.6 mm ID Octadecyl 4-PW and Phenyl-5PW RP columns
*needs cartridge holder