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HIGHLIGHTS
TSKgel OligoDNA RP and TSKgel TMS-250 both incorporate
spherical porous silica with 250 Å pores to allow unhindered access
by large oligonucleotides and proteins respectively
TSKgel OligoDNA RP contains a monomeric C18 bonded phase that
is not endcapped
TSKgel TMS-250 is exhaustively and repeatedly reacted with
trimethyl silyl groups. Standard nomenclature designates the
bonded phase as C1
TSKgel OligoDNA RP is available in 4.6 mm ID and 7.8 mm ID (both
15 cm length), while TSKgel TMS-250 is only available in 4.6 mm ID
x 7.5 cm L
APPLICATIONS
TSKgel OLIGODNA RP
Ideal for the purification and analysis of oligonucleotides (up to
500-mer), RNAs, and DNA fragments
Possesses high-resolving power for octamers of similar sequence
Proteins exhibit sharp peaks relative to wide pore C8 or C18 columns
TSKgel TMS-250
Recommended for the analysis of proteins
The “wide-pore” TMS-250 packing can accommodate large proteins,
such as aldolase (158,000 Da).
RP Columns for Biomolecules TSKgel OLIGODNA RP / TMS-250
figure 8
High Resolution Protein Separation on TSKgel TMS-250
High resolution protein separation on
TSK-gel TMS-250
Minutes
0
30
15
2
1
3
4
7
5
6
Sample:
Elution:
Flow Rate:
Detection:
TSKgel
TMS-250, 4.6 mm ID x 7.5 cm L
5
µ
g each of: 1. ribonuclease A,
2. cytochrome C, 3. lysozyme,
4. bovine serum albumin, 5. aldolase,
6. carbonic anhydrase, 7. ovalbumin
60 min (TMS-250) linear gradient from
20% to 95% CH
3
CN in 0.05% TFA, pH 2.2
0.61 mL/min
UV @ 220 nm
Column: TSKgel TMS-250, 4.6 mm ID x 7.5 cm L;
Sample: 5 μg each of: 1. ribonuclease A, 2. cytochrome C, 3. lysozyme,
4. bovine serum albumin, 5. aldolase, 6. carbonic anhydrase, 7. ovalbumin;
Elution: 60 min (TMS-250) linear gradient from 20% to 95% CH
3
CN in 0.05%
TFA, pH 2.2; Flow Rate: 0.61 mL/min; Detection: UV@220 nm
ORDERING INFORMATION
Part #
Description
ID Length Particle
Number
Flow rate (mL/min)
Maximum
(mm)
(cm)
size (µm)
theoretical
Range
Max.
pressure
plates
drop (MPa)
TSKgel Stainless Steel Colum s
13352 OligoDNA RP, 250 Å
4.6
15.0
5
7,000 0.6 - 1.0
1.2
12.0
13353 OligoDNA RP, 250 Å
7.8
15.0
5
7,000 2.0 - 3.0
3.2
12.0
07190 TMS-250, 250 Å
4.6
7.5
10
1,500 0.5 - 0.8
1.0
2.0