SOLUTIONS

ToyoScreen

ToyoScreen Columns 

In manufacturing, chromatography unit operations are some of the most critical in the purification of a new recombinant therapeutic protein. In many cases two to four chromatographic steps are needed to achieve the desired purity level for the target molecule. Each step can require up to multi-hundred and perhaps even thousands of liters of a specific resin for success.

The first part of the development process is the laboratory scale screening and selection of a resin. Due to the diversity in available ligand chemistries and base matrices offered by different vendors (e.g., agarose, methacrylate, styrene/divinylbenzene etc.) it is prudent at this stage to screen as many resins as possible. A thorough evaluation is a necessity as each target molecule has very different physical and chromatographic properties. Very often a resin that worked in the past for a similar molecule will not work as effectively for the new target molecule.


Historically, resin screening was accomplished by manually packing various bulk resins into small columns requiring a significant investment in time and cost. In order to improve the efficiency of resin screening experiments, pre-packed ToyoScreen columns containing the most popular Toyopearl resins, now provide a low cost and convenient alternative for the evaluation of Toyopearl ligand chemistries.


Screening of TOYOPEARL HIC Resins with Protein Standards 

toyoscreen_fig1.png 
Resins:             A. TOYOPEARL Ether-650M B. TOYOPEARL PPG-600M
                          C. TOYOPEARL Phenyl-650M D. TOYOPEARL Butyl-650M
                          E. TOYOPEARL SuperButyl-550C F. TOYOPEARL Hexyl-650C
Column size:    ToyoScreen, 1 mL
Mobile phase:  Buffer A: 0.1 mol/L phosphate buffer + 1.8 mol/L sodium sulfate, pH 7.0 
                          Buffer B: 0.1 mol/L phosphate buffer, pH 7.0
Gradient:          30 min linear gradient buffer A to buffer B
Flow rate:         1 mL/min
Samples:          ribonuclease A, lysozyme, α-chymotrypsinogen,  1 mg/mL each 

 

Effect of eluent pH on chromatogram (separation of cell culture supernatant (Anti-TSH)) 

toyoscreen_fig2.png 
Resin:               TOYOPEARL Phenyl-650M
Column size:   ToyoScreen, 1 mL
Mobile phase:  Buffer A: 0.1 mol/L phosphate buffer + 1.8 mol/L ammonium sulfate,
                          pH 7.0 Buffer B: 0.1 mol/L phosphate buffer, pH 7.0
Gradient:          30 min linear gradient, 30 CV
Flow rate:         1 mL/min
Injection vol.:  200 µL
Samples:          cell culture supernatant (× 4 diluted) (antibody: Anti-TSH) 

 

toyoscreen.png

Ion Exchange Chromatography
Cation Exchange

0021396 ToyoScreen IEC Mix Pack, 1 mL
(GigaCap Q-650M/ CM-650M/ S-650M, SuperQ-650M, Q--600C AR)
1 mL x 6 Grades x 1 each
0021397 ToyoScreen IEC Mix Pack, 5 mL
(GigaCap Q-650M/ CM-650M/ S-650M, SuperQ-650M, Q--600C AR)
5 mL x 6 Grades x 1 each
0021394 ToyoScreen IEC Cation Mix Pack, 1 mL
(CM-650M, SP-650M, SP-550C, GigaCap CM-650M/ S-650M)
1 mL x 5 Grades 
0021395 ToyoScreen IEC Cation Mix Pack, 5 mL
(CM-650M, SP-650M, SP-550C, GigaCap CM-650M/ S-650M)
5 mL x 5 Grades 
0023472 ToyoScreen Sulfate-650F, 1 mL 1 mL x 6 each
0023473 ToyoScreen Sulfate-650F, 5 mL 5 mL x 6 each
0021868 ToyoScreen GigaCap S-650M, 1 mL 1 mL x 6 each   
0021869 ToyoScreen GigaCap S-650M, 5 mL 5 mL x 6 each  
0021368 ToyoScreen SP-650M, 1 mL 1 mL x 6 each  
0021369 ToyoScreen SP-650M, 5 mL 5 mL x 6 each 
0021370 ToyoScreen SP-550C, 1 mL 1 mL x 6 each  
0021371 ToyoScreen SP-550C, 5 mL 5 mL x 6 each 
0021870 ToyoScreen MegaCap II SP-550EC, 1 mL 1 mL x 6 each 
0021871 ToyoScreen MegaCap II SP-550EC, 5 mL 5 mL x 6 each 
0021951 ToyoScreen GigaCap CM-650M, 1 mL 1 mL x 6 each  
0021952 ToyoScreen GigaCap CM-650M, 5 mL 5 mL x 6 each 
0021366 ToyoScreen CM-650M, 1 mL 1 mL x 6 each 
0021367 ToyoScreen CM-650M, 5 mL 5 mL x 6 each 

Ion Exchange Chromatography
Anion Exchange

0021392 ToyoScreen IEC Anion Mix Pack, 1 mL
(DEAE-650M, SuperQ-650M, QAE-550C, GigaCap Q-650M, Q-600C AR)
1 mL x 5 Grades
0021393 ToyoScreen IEC Anion Mix Pack, 5 mL
(DEAE-650M, SuperQ-650M, QAE-550C, GigaCap Q-650M, Q-600C AR)
5 mL x 5 Grades 
0023443 ToyoScreen NH2-750F, 1 mL 1 mL x 6 each 
0023444 ToyoScreen NH2-750F, 5 mL 5 mL x 6 each 
0022873 ToyoScreen GigaCap DEAE-650M, 1 mL 1 mL x 6 each 
0022872 ToyoScreen GigaCap DEAE-650M, 5 mL 5 mL x 6 each 
0021859 ToyoScreen GigaCap Q-650M, 1 mL 1 mL x 6 each
0021860 ToyoScreen GigaCap Q-650M, 5 mL 5 mL x 6 each
0021992 ToyoScreen Q-600C AR, 1 mL 1 mL x 6 each
0021993 ToyoScreen Q-600C AR, 5 mL 5 mL x 6 each 
0021360 ToyoScreen DEAE-650M, 1 mL 1 mL x 6 each
0021361 ToyoScreen DEAE-650M, 5 mL 5 mL x 6 each
0021362 ToyoScreen SuperQ-650M, 1 mL 1 mL x 6 each 
0021363 ToyoScreen SuperQ-650M, 1 mL 5 mL x 6 each
0021364 ToyoScreen QAE-550C, 1 mL 1 mL x 6 each
0021365 ToyoScreen QAE-550C, 5 mL 5 mL x 6 each

Mixed Mode Chromatography

0022824 ToyoScreen Mx-Trp-650M, 1 mL 1 mL x 6 each
0022825 ToyoScreen Mx-Trp-650M, 5 mL 5 mL x 6 each 

Hydrophobic Interaction Chromatography

0021398 ToyoScreen HIC Mix Pack, 1 mL
(PPG-600M, Butyl-600M/-650M, Phenyl-600M/.650M, Hexyl-650C)
1 mL x 6 Grades x 1 each  
0021398 ToyoScreen HIC Mix Pack, 5 mL
(PPG-600M, Butyl-600M/-650M, Phenyl-600M/.650M, Hexyl-650C)
5 mL x 6 Grades x 1 each   
0021372 ToyoScreen Ether-650M, 1 mL 1 mL x 6 each
0021373 ToyoScreen Ether-650M, 5 mL 5 mL x 6 each
0021380 ToyoScreen PPG-600M, 1 mL 1 mL x 6 each  
0021381 ToyoScreen PPG-600M, 1 mL 5 mL x 6 each 
0021892 ToyoScreen Phenyl-600M, 1 mL 1 mL x 6 each
0021893 ToyoScreen Phenyl-600M, 5 mL 5 mL x 6 each
0021374 ToyoScreen Phenyl-650M, 1 mL 1 mL x 6 each 
0021375 ToyoScreen Phenyl-650M, 5 mL 5 mL x 6 each 
0021376 ToyoScreen Butyl-650M, 1 mL 1 mL x 6 each 
0021377 ToyoScreen Butyl-650M, 5 mL 5 mL x 6 each 
0021494 ToyoScreen Butyl-600M, 1 mL 1 mL x 6 each 
0021495 ToyoScreen Butyl-600M, 1 mL 5 mL x 6 each 
0021382 ToyoScreen SuperButyl-550C, 1 mL 1 mL x 6 each
0021383 ToyoScreen SuperButyl-550C, 5 mL 5 mL x 6 each 
0021378 ToyoScreen Hexyl-650C, 1 mL 1 mL x 6 each 
0021379 ToyoScreen Hexyl-650C, 1 mL 5 mL x 6 each 


Affinity Chromatography

0023494 ToyoScreen AF-rProtein L-650F, 1 mL 1 mL x 5 each
0023495 ToyoScreen AF-rProtein L-650F, 5 mL 5 mL x 1 each
0023496 ToyoScreen AF-rProtein L-650F, 5 mL 5 mL x 5 each
0023430 ToyoScreen AF-rProtein A HC-650F, 1 mL 1 mL x 5 each
0023431 ToyoScreen AF-rProtein A HC-650F, 5 mL 5 mL x 1 each
0023432 ToyoScreen AF-rProtein A HC-650F, 5 mL 5 mL x 5 each
0022809 ToyoScreen AF-rProtein A-650F, 1 mL 1 mL x 5 each
0022810 ToyoScreen AF-rProtein A-650F, 5 mL 5 mL x 1 each 
0022811 ToyoScreen AF-rProtein A-650F, 5 mL 5 mL x 5 each
0021384 ToyoScreen AF-Chelate-650M, 1 mL 1 mL x 6 each 
0021385 ToyoScreen AF-Chelate-650M, 5 mL 5 mL x 6 each 
0021390 ToyoScreen AF-Heparin HC-650M, 1 mL 1 mL x 6 each 
0021391 ToyoScreen AF-Heparin HC-650M, 5 mL 5 mL x 6 each 
0021388 ToyoScreen AF-Red-650M, 1 mL 1 mL x 6 each 
0021389 ToyoScreen AF-Red-650M, 1 mL 5 mL x 6 each 

ToyoScreen Accessories

0021400 ToyoScreen Column Holder 1 mL x 6 each