SOLUTIONS

RoboColumns

ToyoScreen RoboColumns 

ToyoScreen RoboColumns are miniaturized chromatographic columns pre-packed with TOYOPEARL ion exchange, mixed-mode, hydrophobic interaction or affinity media. Available in different volumes, they are designed to operate with a robotic liquid handling system, such as the Freedom EVO® from TECAN. This approach allows automated high-throughput, small-scale bio-chromatographic separations of protein samples by running up to eight individual columns simultaneously. ToyoScreen RoboColumns are packed with TOYOPEARL to our specifications by Repligen and are supplied in strips of 8 columns.

ToyoScreen RoboColumns can be used in a wide range of applications, including individual and parallel resin screening, optimization of separation conditions, scale-down experiments, as well as high throughput sample preparation. Binding and elution conditions, washing parameters, etc. can be investigated to explore the design space of the particular molecule’s purification process.

Optimization of anion exchange conditions 

Fig1_RoboColumns.png 
Elution profile of a protein A capture eluate on ToyoScreen RoboColumns packed with TOYOPEARL SuperQ-650M at various conditions. Data kindly provided by T. Schröder, Repligen GmbH. 

 

Robocolumns.png

ToyoScreen RoboColumns for fast automated screening of resins

0045099  Array Plate


Gel Filtration / Desalting

0045071 RoboColumn HW-40F 0.2 mL x 8 columns
0045072 RoboColumn HW-40F 0.6 mL x 8 columns


Ion Exchange Chromatography

0045027 RoboColumn Sulfate-650F 0.2 mL x 8 columns 
0045028 RoboColumn Sulfate-650F 0.6 mL x 8 columns
0045023 RoboColumn GigaCap S-650S  0.2 mL x 8 columns 
0045024 RoboColumn GigaCap S-650S 0.6 mL x 8 columns
0045001 RoboColumn GigaCap S-650M 0.2 mL x 8 columns 
0045002 RoboColumn GigaCap S-650M 0.6 mL x 8 columns
0045025 RoboColumn GigaCap Q-650S 0.2 mL x 8 columns 
0045026 RoboColumn GigaCap Q-650S 0.6 mL x 8 columns
0045003 RoboColumn GigaCap Q-650M 0.2 mL x 8 columns 
0045004 RoboColumn GigaCap Q-650M 0.6 mL x 8 columns
0045005 RoboColumn GigaCap CM-650M 0.2 mL x 8 columns 
0045006 RoboColumn GigaCap CM-650M 0.6 mL x 8 columns
0045007 RoboColumn GigaCap DEAE-650M 0.2 mL x 8 columns 
0045008 RoboColumn GigaCap DEAE-650M 0.6 mL x 8 columns
0045011 RoboColumn Q-600C AR 0.2 mL x 8 columns 
0045012 RoboColumn Q-600C AR 0.6 mL x 8 columns
0045021 RoboColumn NH2-750F 0.2 mL x 8 columns 
0045022 RoboColumn NH2-750F 0.6 mL x 8 columns


Mixed Mode Chromatography

0045051 RoboColumn MX-Trp-650M 0.2 mL x 8 columns
0045052 RoboColumn MX-Trp-650M 0.6 mL x 8 columns
0045053 RoboColumn Ca++Pure-HA 0.2 mL x 8 columns
0045054 RoboColumn Ca++Pure-HA 0.6 mL x 8 columns


Hydrophobic Interaction Chromatography

0045031 RoboColumn Phenyl-600M 0.2 mL x 8 columns
0045032 RoboColumn Phenyl-600M 0.6 mL x 8 columns
0045033 RoboColumn Butyl-600M 0.2 mL x 8 columns
0045034 RoboColumn Butyl-600M 0.6 mL x 8 columns
0045035 RoboColumn PPG-600M 0.2 mL x 8 columns
0045036 RoboColumn PPG-600M 0.6 mL x 8 columns
0045037 RoboColumn Phenyl-650M 0.2 mL x 8 columns
0045038 RoboColumn Phenyl-650M 0.6 mL x 8 columns
0045089 RoboColumn Butyl-650M 0.2 mL x 8 columns
0045090 RoboColumn Butyl-650M 0.6 mL x 8 columns
0045091 RoboColumn Hexyl-650C 0.2 mL x 8 columns 
0045092 RoboColumn Hexyl-650C 0.6 mL x 8 columns

Affinity Chromatography

0045061 RoboColumn AF-rProtein A-650F 0.2 mL x 8 columns
0045062 RoboColumn AF-rProtein A-650F 0.6 mL x 8 columns
0045063 RoboColumn AF-rProtein A HC-650F 0.2 mL x 8 columns
0045064 RoboColumn AF-rProtein A HC-650F 0.6 mL x 8 columns
0045065 RoboColumn AF-rProtein L-650F 0.2 mL x 8 columns
0045066 RoboColumn AF-rProtein L-650F 0.6 mL x 8 columns