ToyoScreen RoboColumns 

ToyoScreen RoboColumns are miniaturized chromatographic columns pre-packed with TOYOPEARL ion exchange, mixed-mode, hydrophobic interaction or affinity media. Available in different volumes, they are designed to operate with a robotic liquid handling system, such as the Freedom EVO® from TECAN. This approach allows automated high-throughput, small-scale bio-chromatographic separations of protein samples by running up to eight individual columns simultaneously. ToyoScreen RoboColumns are packed with TOYOPEARL to our specifications by Repligen and are supplied in strips of 8 columns.

ToyoScreen RoboColumns can be used in a wide range of applications, including individual and parallel resin screening, optimization of separation conditions, scale-down experiments, as well as high throughput sample preparation. Binding and elution conditions, washing parameters, etc. can be investigated to explore the design space of the particular molecule’s purification process.

Optimization of anion exchange conditions 

Elution profile of a protein A capture eluate on ToyoScreen RoboColumns packed with TOYOPEARL SuperQ-650M at various conditions. Data kindly provided by T. Schröder, Atoll GmbH.