SOLUTIONS

TOYOPEARL HW-75

TOYOPEARL HW-75

TOYOPEARL HW-75 is a hydroxylated methacrylic polymer with a pore size of over 100 nm (>1000 A) for size exclusion chromatography of biomolecules between 500,000-50,000,000 Da.

TOYOPEARL HW-75 is available in two particle sizes, 30 µm (S-grade) and 45 µm (F-grade).

 

Structure of TOYOPEARL HW Resins

sec_resins_structure.png

Comparison of resolution on different particle sizes of TOYOPEARL HW-55 resin

sec_resins_resolution.png
Resin:              TOYOPEARL HW-55
Column size:  26 mm ID × 70 cm
Mobile phase: 33.3 mmol/L phosphate buffer, pH 7.0, 0.2 mol/L NaCI
Flow rate:        20 cm/hr (1.77 mL/min)
Detection:       UV @ 280 nm
Temperature: 25 °C
Injection vol.: 1 mL
Samples:         1. thyroglobulin (0.3%), 2. gamma-globulin (0.3%),
                         3. β-lactoglobulin (0.3%), 4. cytochrome C (0.1%)

 

Ordering Information

P/N Description Dimension
0019816 TOYOPEARL HW-75F 150 mL
0007469 TOYOPEARL HW-75F 500 mL
0014691 TOYOPEARL HW-75F 1 L
0008072 TOYOPEARL HW-75F 5 L