SOLUTIONS

TOYOPEARL HW-50

TOYOPEARL HW-50

TOYOPEARL HW-50 is a hydroxylated methacrylic polymer with a pore size of 12.5 nm (125 A) for size exclusion chromatography of biomolecules between 500-80,000 Da.

TOYOPEARL HW-50 is available in two particle sizes, 30 µm (S-grade) and 45 µm (F-grade)

 

Structure of TOYOPEARL HW Resins

sec_resins_structure.png

Comparison of resolution on different particle sizes of TOYOPEARL HW-55 resin

sec_resins_resolution.png
Resin:              TOYOPEARL HW-55
Column size:  26 mm ID × 70 cm
Mobile phase: 33.3 mmol/L phosphate buffer, pH 7.0, 0.2 mol/L NaCI
Flow rate:        20 cm/hr (1.77 mL/min)
Detection:       UV @ 280 nm
Temperature: 25 °C
Injection vol.: 1 mL
Samples:         1. thyroglobulin (0.3%), 2. gamma-globulin (0.3%),
                         3. β-lactoglobulin (0.3%), 4. cytochrome C (0.1%)

 

Ordering Information

P/N Description Dimension
0019810 TOYOPEARL HW-50F 150 mL
0007453 TOYOPEARL HW-50F 500 mL
0014685 TOYOPEARL HW-50F 1 L
0008060 TOYOPEARL HW-50F 5 L
0019811 TOYOPEARL HW-50S 150 mL
0007455 TOYOPEARL HW-50S 250 mL
0014684 TOYOPEARL HW-50S 1 L
0008059 TOYOPEARL HW-50S 5 L