SOLUTIONS

TSKgel SP-5PW

TSKgel SP-5PW 

TSKgel SP-5PW is a strong cation exchange resin for biomolecule purification. It is composed of highly crosslinked polymethacrylate beads that have been functionalized with sulfopropyl (SP) strong cation exchange groups. TSKgel SP-5PW resins are available in two particle sizes: 30 µm and 20 µm, and ideally suited for high resolution polishing steps.


Structure of TSKgel SP-5PW  

SP5PW_structure.jpg
 

 

Product attributes

Pore size (mean): 100 nm
Particle size (mean): 30 µm
20 µm
Pressure rating: 0.2 Mpa
Shipping buffer: 20% ethanol
pH stability: 2-13
Shelf life: 10 years (estimated)

Selectivity Comparison 

SP-3Pw 
Column:           7.5 mm ID x 7.5 cm L
Mobile phase:  A: 20 mM sodium citrate buffer (pH 3.2)/ethanol = 8/2 (v/v);
                          B: 1.0 mol/L NaCl in 20 mM sodium citrate buffer
                         (pH 3.2)/ethanol = 8/2 (v/v)
Gradient:         60 min linear gradient from Buffer A to Buffer B
Flow rate:        1.0 mL/min
Detection:        UV @ 280 nm
Temperature:  RT
Sample:           1. trypsinogen, 2. insulin, 3. lysozyme; 100 µL (0.5 mg/ml each)
 

 

Ordering Information

P/N Description Dimension
0043282 TSKgel SP-5PW (30) 25 mL
0014716 TSKgel SP-5PW (30) 250 mL
0014717 TSKgel SP-5PW (30) 1 L
0018384 TSKgel SP-5PW (30) 5 L
0043382 TSKgel SP-5PW (20) 25 mL
0014714 TSKgel SP-5PW (20) 250 mL
0014715 TSKgel SP-5PW (20) 1 L
0018435 TSKgel SP-5PW (20) 5 L