SOLUTIONS

TSKgel SP-3PW

TSKgel SP-3PW

TSKgel SP-3PW is a strong cation exchange resin for biomolecule purification. It is composed of highly cross-linked polymethacrylate beads that have been functionalized with sulfopropyl (SP) strong cation exchange groups.

TSKgel SP-3PW resins are best used for high resolution process polishing steps for peptide purifications such as insulin. TSKgel SP-3PW resin is available in 30 µm particle sizes, and has a smaller pore size (25 nm) than the TSKgel SP-5PW (100 nm).

Structure of TSKgel SP-3PW  

SP3PW_structure.jpg 

 

Selectivity Comaprison 

SP-3Pw 
Column:           7.5 mm ID x 7.5 cm L
Mobile phase:  A: 20 mM sodium citrate buffer (pH 3.2)/ethanol = 8/2 (v/v);
                          B: 1.0 mol/L NaCl in 20 mM sodium citrate buffer
                         (pH 3.2)/ethanol = 8/2 (v/v)
Gradient:         60 min linear gradient from Buffer A to Buffer B
Flow rate:        1.0 mL/min
Detection:        UV @ 280 nm
Temperature:  RT
Sample:           1. trypsinogen, 2. insulin, 3. lysozyme; 100 µL (0.5 mg/ml each)
 

 

Ordering Information

P/N Description Dimension
0021976 TSKgel SP-3PW (30) 25 mL
0021977 TSKgel SP-3PW (30) 250 mL
0021978 TSKgel SP-3PW (30) 1 L
0021979 TSKgel SP-3PW (30) 5 L