TOYOPEARL Sulfate-650F

Salt Tolerant Cation Exchanger with High IgG Capacity

TOYOPEARL Sulfate-650F is a new salt tolerant cation exchange resin that offers a high binding capacity for Immunoglobulin G (IgG) across a range of pH values and conductivities. It provides an increased salt tolerance and its selectivity is different than that of most sulfate type cation exchange media currently available.

TOYOPEARL Sulfate-650F is ideal for a range of applications including the capture of proteins from biological feedstock (mammalian cell culture or bacterial feedstock) without dilution and the intermediate or final purification of monoclonal antibodies (mAbs). It is especially suitable for post-Protein A removal of aggregates in bind-elute mode. TOYOPEARL Sulfate-650F provides stronger binding of mAb aggregates than other cation exchange media, resulting in the high resolution separation of monomer and aggregates.

High protein binding capacity at elevated ion strength 
Works within a wide pH range 
mAb aggregate removal in bind-elute mode 
Affinity capturing of selected targets 


Post-Protein A Aggregate Removal 

Sulfate-650 Aggregate Removal
Fractions collected and analyzed by SEC on TSKgel G3000SWXL 


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