SOLUTIONS

TOYOPEARL GigaCap CM-650

TOYOPEARL GigaCap CM-650

TOYOPEARL GigaCap CM-650 is a high capacity cation exchange resin optimized for the high throughput purification of IgG’s. It is composed of polymethacrylate beads that have been chemically modified to provide a higher number of cationic binding sites and functionalized with carboxymethyl (CM) weak cation exchange groups.

TOYOPEARL GigaCap CM-650 exhibits different selectivity than the TOYOPEARL GigaCap S-650 resin. This difference, combined with its unusually high capacity for a weak cation exchange resin, may be exploited for wither mAb or traditional protein purifications. TOYOPEARL GigaCap CM-650 resin is available in 75 µm particle size, which corresponds to the M-grade designation for TOYOPEARL resins.

TOYOPEARL GigaCap CM-650M exhibits typical dynamic binding capacities of 100 g/L for human IgG and maintains its high capacity at increased linear velocities. It has excellent binding and elution kinetics, which results in a more concentrated protein fraction collected during the elution step. TOYOPEARL GigaCap CM-650 media also has excellent caustic stability in 0.5 mol/L NaOH.

Structure of TOYOPEARL GigaCap CM-650M 

GigaCapCM650_structure.jpg 

 

Product attributes

Pore size (mean): < 100 nm
Particle size (mean): 75 µm (C-grade)
Pressure rating: 0.3 Mpa
Shipping buffer: 20% ethanol
pH stability: 3-13
Shelf life: 10 years (estimated)

TOYOPEARL GigaCap CM-650M has unique selectivity

gigacapcm_fig1.png 
Resins:            TOYOPEARL GigaCap CM-650M TOYOPEARL GigaCap S-650M
Column size:   6 mm ID × 4 cm
Mobile phase: Buffer  A: 20 mmol/L phosphate, pH 7.0
                         Buffer  B: 20 mmol/L phosphate + 1.0 mol/L NaCl, pH 7.0
Gradient:        60 min linear gradient from buffer A to buffer B
Flow rate:        212 cm/hr (1.0 mL/min) 
Detection:       UV @ 280 nm
Injection vol.: 25 µL
Samples:         (A) 1. ribonuclease A (5.0 g/L)  2. cytochrome C (1.9 g/L)
                         3. lysozyme (3.8 g/L) 
                         (B) 1. trypsinogen (3.8 g/L) 2. ribonuclease A (5.0 g/L)
                          3. lysozyme (3.8 g/L) 

 

Selectivity comparison between native protein and mono-PEGylated protein on TOYOPEARL GigaCap resins 

gigacaps_fig2.png 
Resins:            TOYOPEARL GigaCap S-650M TOYOPEARL GigaCap CM-650M
Column size:   6 mm ID × 4 cm
Mobile phase: Buffer A: 50 mmol/L phosphate buffer, pH 7.0
                         Buffer B: 50 mmol/L phosphate buffer, pH 7.0 + 0.5 mol/L NaCl
Gradients:       10 minute 100% buffer A 10 minute 100% buffer A 60 minutes
                         0%B to 100%B 60 minutes 0%B to 50%B
Flow rate:        212 cm/hr (1 mL/min)
Samples:         native lysozyme, 5 g/L mono-PEGylated lysozyme, 5 g/L
                         (PEG MW= 5 kDa) 

 

Ordering Information

P/N Description Dimension
0021946 TOYOPEARL GigaCap CM-650M 100 mL
0021947 TOYOPEARL GigaCap CM-650M 250 mL
0021948 TOYOPEARL GigaCap CM-650M 1 L
0021949 TOYOPEARL GigaCap CM-650M 5 L
0021950 TOYOPEARL GigaCap CM-650M 50 L
0045103 MiniChrom TOYOPEARL GigaCap CM-650M, 5 mL 8 mm ID x 100 mm ID
0045005 RoboColumn GigaCap CM-650M 200 x 8 µL
0045006 RoboColumn GigaCap CM-650M 600 x 8 µL
0021951 ToyoScreen GigaCap CM-650M 6 x 1 mL
0021952 ToyoScreen GigaCap CM-650M 6 x 5 mL
0021394 ToyoScreen IEC Cation Mix Pack, (GigaCap CM-650M,
GigaCap S-650M, CM-650M, SP-650M, SP-550M)
5 x 1 mL
0021395 ToyoScreen IEC Cation Mix Pack, (GigaCap CM-650M,
GigaCap S-650M, CM-650M, SP-650M, SP-550M)
5 x 5 mL
0021396 ToyoScreen IEC Mix Pack, (GigaCap CM-650M,
GigaCap S-650M, GigaCap Q-650M, Q-600C AR,
SuperQ-650M, SP-550C)
6 x 1 mL
0021397 ToyoScreen IEC Mix Pack, (GigaCap CM-650M,
GigaCap S-650M, GigaCap Q-650M, Q-600C AR,
SuperQ-650M, SP-550C)
6 x 5 mL