SOLUTIONS

TSKgel DEAE-5PW

TSKgel DEAE-5PW

TSKgel DEAE-5PW is a weak anion exchange resin for biomolecule purification. The resin is composed of highly crosslinked polymethacrylate beads that have been functionalized with tertiary amine groups, namely diethylaminoethyl groups. TSKgel DEAE-5PW resins are best used for high resolution process polishing steps. They are available in two particle sizes: 30 µm and 20 µm.

TSKgel DEAE-5PW resins have high chemical and mechanical stability, allowing cleaning-in-place (CIP) and high flow rates. TSKgel PW resins vary from TOYOPEARL resins by having a higher degree of crosslinking which is necessitated by the higher flow pressures needed when using smaller particles for chromatography.

Because similarly functionalized TSKgel PW and TOYOPEARL resin types have the same backbone polymer chemistry, the selectivity for proteins and impurities remains the same.

Structure of TSKgel DEAE-5PW 

DEAE5PW_structure.jpg 

 

Product attributes

Pore size (mean): < 100 nm
Particle size (mean): 30 µm
20 µm
Pressure rating: 2 Mpa
Shipping buffer: 20% ethanol
pH stability: 2-13
Shelf life: 10 years (estimated)

Selectivity Comparison of Anion Exchange Resins 

deae-5pw_fig1.png 
Resins: A. TOYOPEARL GigaCap Q-650S B. Competitor Q  C. Competitor Q  D. TOYOPEARL SuperQ-650S E. TSKgel SuperQ-5PW (30) F. TSKgel SuperQ-5PW (20) G. TOYOPEARL DEAE-650S H. TSKgel DEAE-5PW (30) I. TSKgel DEAE-5PW (20)
Column size: 7.5 mm ID × 7.5 cm 
Mobile phase: Buffer A: 50 mmol/L Tris-HCl, pH 8.5 
Buffer B: buffer A + 1.0 mol/L NaCl
Gradient: 0-100% buffer B (120 min)
Flow rate: 136 cm/hr (1.0 mL/min) 
Detection: UV @ 280 nm
Injection vol.: 100 µL
Samples: transferrin, 2.9 g/L ovalbumin, 6.5 g/L trypsin inhibitor, 10.0 g/L 

 

Ordering Information

P/N Description Dimension
0043281 TSKgel DEAE-5PW (30) 25 mL
0014712 TSKgel DEAE-5PW (30) 250 mL
0014713 TSKgel DEAE-5PW (30) 1 L
0018370 TSKgel DEAE-5PW (30) 5 L
0043381 TSKgel DEAE-5PW (20) 25 mL
0014710 TSKgel DEAE-5PW (20) 250 mL
0014711 TSKgel DEAE-5PW (20) 1 L
0018436 TSKgel DEAE-5PW (20) 5 L