SOLUTIONS

TOYOPEARL Q-600C AR

TOYOPEARL Q-600C AR

TOYOPEARL Q-600C AR is a strong anion exchange resin designed to be more alkaline resistant (AR) than TOYOPEARL QAE-550C resin. It has a slightly larger pore diameter than TOYOPEARL QAE-550C (75 nm vs. 50 nm), but its ligand attachment chemistry creates significantly more dynamic binding capacity (up to 3X higher) than TOYOPEARL QAE-550C resin. TOYOPEARL Q-600C AR tolerates caustic cleaning solutions up to 1.0 mol/L NaOH well with essentially no change in DBC. It has excellent elution kinetics and is slightly more retentive than TOYOPEARL QAE-550C.

The resin is composed of a base material of hydroxylated methacrylic polymer beads that have been functionalized with proprietary quaternary amine strong anion exchange groups. TOYOPEARL QAE-550C can be used for the high throughput capture of proteins, including monoclonal antibodies. It is available in 100 µm particle size, corresponding to the C-grade designation.

Structure of TOYOPEARL Q-600C AR 

QAE550_structure.jpg 

 

Product attributes

Pore size (mean): < 75 nm
Particle size (mean): 100 µm (C-grade)
Pressure rating: 0.3 Mpa
Shipping buffer: 20% ethanol
pH stability: 2-13
Shelf life: 10 years (estimated)

Stability of TOYOPEARL Q-600C AR resin after exposure to 1 mol/L NaOH 

q600ar_fig1.png 
Resin:              TOYOPEARL Q-600C AR
Column size:   6.0 mm ID × 4 cm
Mobile phase: Buffer A: 0.05 mol/L Tris-HCl  buffer, pH 8.5                           
                         Buffer B: 0.05 mol/L Tris-HCl  buffer + 1.0 mol/L NaCl, pH 8.5 
Gradient:        60 min linear gradient from buffer A to buffer B
Flow rate:       212 cm/hr (1.0 mL/min)
Detection:       UV @ 280 nm
Samples:         1. ovalbumin 2. soybean trypsin inhibitor

 

Ordering Information

P/N Description Dimension
0021985 TOYOPEARL Q-600C AR 100 mL
0021986 TOYOPEARL Q-600C AR 250 mL
0021987 TOYOPEARL Q-600C AR 1 L
0021988 TOYOPEARL Q-600C AR 5 L
0021981 TOYOPEARL Q-600C AR 50 L
0021992 ToyoScreen Q-600C AR 6 x 1 mL
0021993 ToyoScreen Q-600C AR 6 x 5 mL
0021392 ToyoScreen IEC Anion Mix Pack, (GigaCap Q-650M,
Q-600C AR, DEAE-650M, SuperQ-650M, QAE-550C)
5 x 1 mL
0021393 ToyoScreen IEC Anion Mix Pack, (GigaCap Q-650M,
Q-600C AR, DEAE-650M, SuperQ-650M, QAE-550C)
5 x 5 mL
0021392 ToyoScreen IEC Anion Mix Pack, (GigaCap Q-650M,
Q-600C AR, DEAE-650M, SuperQ-650M, QAE-550C)
5 x 1 mL
0021393 ToyoScreen IEC Anion Mix Pack, (GigaCap Q-650M,
Q-600C AR, DEAE-650M, SuperQ-650M, QAE-550C)
5 x 5 mL