SOLUTIONS

TOYOPEARL GigaCap DEAE-650

TOYOPEARL GigaCap DEAE-650M

TOYOPEARL GigaCap DEAE-650M is a high capacity anion exchange resin composed of polymethacrylate beads that have been chemically modified to provide a higher number of anionic binding sites and functionalized with diethyl amino ethyl (DEAE) weak anion exchange groups.

TOYOPEARL GigaCap DEAE-650 can be used for high throughput capture and intermediate purification of blood products and other proteins. It is available in 75 µm particle size (M-grade). It exhibits typical dynamic binding capacities of 165 g/L for BSA and maintains the high capacity even at increased linear velocities. It also has excellent binding and elution kinetics, which results in a more concentrated protein fraction collected during the elution step.

TOYOPEARL GigaCap DEAE-650M 

GigaCapDEAE650_structure.jpg 

 

Product attributes

Pore size (mean): < 100 nm
Particle size (mean): 75 µm (M-grade)
Pressure rating: 0.3 Mpa
Shipping buffer: 20% ethanol
pH stability: 3-13
Shelf life: 10 years (estimated)

Selectivity Comparison 

gigacapdeae_fig1.png 

Resins:             TOYOPEARL GigaCap DEAE-650M
                          TOYOPEARL GigaCap Q-650M
                          Competitor DEAE
Column size:    7.5 mm ID × 7.5 cm
Mobile phase:  Buffer A: 50 mmol/L Tris-HCl, pH 8.5
                          Buffer B: buffer A + 1.0 mol/L NaCl, pH 8.5
Gradient:         120 minutes, 0 - 100% B
Flow rate:        136 cm/hr (1.0 mL/min)
Detection:         UV @ 280 nm
Temperature:   ambient
Injection vol.:  100 µL
Samples:          1. transferrin, 2.9 g/L  2. ovalbumin, 6.5 g/L 3. trypsin inhibitor, 10.0 g/L
Sample load:   1.94 mg total protein 

 

Ordering Information

P/N Description Dimension
0022865 TOYOPEARL GigaCap DEAE-650M 100 mL
0022866 TOYOPEARL GigaCap DEAE-650M 250 mL
0022867 TOYOPEARL GigaCap DEAE-650M 1 L
0022868 TOYOPEARL GigaCap DEAE-650M 5 L
0045106 MiniChrom TOYOPEARL GigaCap DEAE-650M, 5 mL 8 x 100 mm
0045007 RoboColumn GigaCap DEAE-650M 8 x 200 µL
0045008 RoboColumn GigaCap DEAE-650M 8 x 600 µL
0022872 ToyoScreen GigaCap DEAE-650M 6 x 1 mL
0022873 ToyoScreen GigaCap DEAE-650M 6 x 5 mL