SOLUTIONS

Ca++Pure-HA Performance Data

Hydroxyapatite Chromatography

Ca⁺⁺Pure-HA resin has been sintered at high temperatures for increased mechanical and pH stability, allowing it to withstand the rigors of industrial-scale applications. Ca⁺⁺Pure-HA has a demonstrated dynamic binding capacity (DBC), at 5% breakthrough, of 55 mg mAb/mg resin at 5 minute residence time. 

Mechanical Stability
ca--pure-mechanicalstability.jpg

Caustic Stability
ca--pure-causticstability.jpg
Resin:                          Ca⁺⁺Pure-HA
Column size:               1.1 cm ID × 19.3 cm (dry pack)
                                    2.2 cm ID × 20.3 cm (dry pack)
                                    4.4 cm ID × 19.3 cm (dry pack)
                                    2.2 cm ID × 20.6 cm (wet pack)
                                   10.0 cm ID × 21.3 cm (wet pack)
Mobile phase:              5 mmol/L sodium phosphate, pH 7.2
Linear velocity:            as noted
Detection:                    pressure (MPa)

Dynamic Binding Capacity - Human IgG
ca--pure-dbc-human-igg.jpg

Resin:                      Ca⁺⁺Pure-HA
Column size:            6.6 cm ID × 3 cm (1 mL)
Mobile phase:          5 mmol/L sodium phosphate, pH 6.8
Flow rate:                 5 min residence time: 0.21 mL/min (36 cm/hr)
                                 3.5 min residence time: 0.3 mL/min (53 cm/hr)
                                 2 min residence time: 0.52 mL/min (91 cm/hr)
                                1 min residence time: 1.0 mL/min (180 cm/hr)
Detection:                UV @ 280 nm (10% breakthrough)
Sample:                   lysozyme, 1.0 g/L

Dynamic Binding Capacity - Lysozyme 
ca--pure-dbc-lysozyme.jpg

Resin:                     Ca⁺⁺Pure-HA
Column size:          6.6 cm ID × 3 cm (1 mL)
Mobile phase:        10 mmol/L sodium phosphate, pH 6.5
Flow rate:                5 min residence time: 0.21 mL/min (36 cm/hr)
                                2 min residence time: 0.52 mL/min (91 cm/hr)
Detection:               UV @ 280 nm (10% breakthrough)
Sample:                  human IgG, 1.0 g/L

1 min 2 min 3.5 min 5 min
Lysozyme 24.9 g/L 24.9 g/L 25.0 g/L 25.1 g/L
Human IgG  32.4 g/L N/A N/A 51.6 g/L