Hydroxyapatite Chromatography
Ca⁺⁺Pure-HA is a hydroxyapatite resin used in the chromatographic separation of biomolecules. Key applications for use of Ca⁺⁺Pure-HA include the purification of monoclonal and polyclonal antibodies, including IgG, IgA, IgM, antibody fragments, fusion- and phosphoproteins, and the separation of single-stranded and double-stranded DNA.
Ca⁺⁺Pure-HA resin has been sintered at high temperatures for increased mechanical and pH stability, allowing it to withstand the rigors of industrial-scale applications. Ca⁺⁺Pure-HA has a demonstrated dynamic binding capacity (DBC), at 5% breakthrough, of 55 mg mAb/mg resin at 5 minute residence time.
Ca⁺⁺Pure-HA provides exceptional separation properties and unequalled selectivity and resolution for multiple classes of biomolecules. The robust nature of Ca⁺⁺Pure-HA offers the flexibility to use this resin at any stage in a process from capture to final polishing.
| Structure of Ca++Pure-HA |
 |
Product Attributes
| Particle size (mean) |
39 µm |
| Pressure rating |
10 MPa |
| Shipped as |
dry powder |
| pH stability |
6.5 - 14.0 |
| Shelf life |
10 years |
More information on stabillity and dynamic binding capacity for Ca++Pure-HA »