SOLUTIONS

TSKgel Phenyl-5PW

TSKgel Phenyl-5PW

TSKgel Phenyl-5PW is based on a highly crosslinked 100 nm (1000 Å) pore size polymethacrylate base material bonded with phenyl groups. The TSKgel Phenyl-5PW column is stable in either acid or caustic cleaning regimens and provides excellent access to larger molecules with low diffusion coefficients.

TSKgel Phenyl-5PW is less hydrophobic than Ether-5PW and the best choice for the separation of proteins with low hydophobicity.

 

Structure of TOYOPEARL Phenyl-5PW resins 

Phenyl5PW_structure.png
 

 

Product attributes

Pore size (mean): 100 nm
Particle size (mean): 20 µm
30 µm
Pressure rating: 0.2 Mpa
Shipping buffer: 20% ethanol
pH stability: >1-13
Shelf life: 10 years (estimated)

Typical dynamic binding capacities for humanized mAb

HIC_fig2.png 
                                                Binding capacitiy (mg/mL - gel)
                                                (10% breakthrough)
Phenyl-650M                                           35.2
Butyl-650M                                             42.1
PPG-600M                                              44.8
Butyl-600M                                             48.5 

Selectivity of TOYOPEARL HIC resins

HIC_fig3.png
Samples: 1. Ribonuclease A, 2. Lysozyme, 3. α- Chymotrypsinogen

Ordering Information

P/N Description Dimension
0043277 TSKgel Phenyl-5PW (20) 25 mL
0014718 TSKgel Phenyl-5PW (20) 250 mL
0014719 TSKgel Phenyl-5PW (20) 1 L
0018438 TSKgel Phenyl-5PW (20) 5 L
0043177 TSKgel Phenyl-5PW (30) 25 mL
0014720 TSKgel Phenyl-5PW (30) 250 mL
0014721 TSKgel Phenyl-5PW (30) 1 L
0017210 TSKgel Phenyl-5PW (30) 5 L