SOLUTIONS

TSKgel Ether-5PW

TSKgel Ether-5PW

TSKgel Ether-5PW is based on a highly crosslinked 100 nm (1000 Å) pore size polymethacrylate base material bonded with polyether groups. TSKgel Ether-5PW provides the lowest hydrophobicity of the five HIC ligands offered by Tosoh Bioscience. It is useful for the separation of very hydrophobic molecules such as membrane proteins. It is typically used for high performance chromatography and polishing steps. It has the same selectivity as the larger particle TOYOPEARL Ether-650 products.

TSKgel Ether-5PW is less hydrophobic than Phenyl-5PW and the best choice for the separation of very hydrophobic proteins or monoclonal antibodies.

Structure of TSKgel Ether-5PW resins 

Ether5PW_structure.png
 

 

Product attributes

Pore size (mean): 100 nm
Particle size (mean): 30 µm
20 µm
Pressure rating: 0.2 Mpa
Shipping buffer: 20% ethanol
pH stability: >1-13
Shelf life: 10 years (estimated)

Purification of mAbs from mouse ascites fluid on TSKgel Ether-5PW and TOYOPEARL Ether-650M

ether5pw_fig2.png
 
Resins:             A: TSKgel Ether-5PW (prepacked HPLC column)
                          B: Toyopearl Ether-650M
Column size:    7.5 mm ID × 7.5 cm
Mobile phase: Buffer A: 1.5 mol/L (NH4)2SO4 + 0.1mol/L phosphate, pH 7.0
                          Buffer B: 0.1 mol/L phosphate, pH 7.0
Gradient:         60 min linear gradient from buffer A to B
Flow rate:        136 cm/hr (1.0 mL/min)
Detection:        UV @ 280 nm
Temperature: ambient
Sample:           A: 1.5 mg/100 µL anti-chicken 14 kDa lectin
                         B: 0.76 mg/50 µL diluted ascites fluid
Load volume: 150 µL

Ordering Information

P/N Description Dimension
0043276 TSKgel Ether-5PW (20) 25 mL
0016052 TSKgel Ether-5PW (20) 250 mL
0016053 TSKgel Ether-5PW (20) 1 L
0018437 TSKgel Ether-5PW (20) 5 L
0043176 TSKgel Ether-5PW (30) 25 mL
0016050 TSKgel Ether-5PW (30) 250 mL
0016051 TSKgel Ether-5PW (30) 1 L
0018439 TSKgel Ether-5PW (30) 5 L