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TOYOPEARL SuperButyl-550C

TOYOPEARL SuperButyl-550C

TOYOPEARL SuperButyl-550C is based on a 50 nm (500 Å) pore size polymethacrylate base material bonded with C4 groups. It has the second highest hydrophobicity of the TOYOPEARL HIC resins. Its pore size is the smallest of our three butyl resins.

Primary applications are for the separation of smaller proteins. Depending on the feedstock's molecular size profile, it is also useful in negative or flow through chromatography. It can be used for capture and intermediate purification process steps.

 

Structure of TOYOPEARL SuperButyl-550C  

SuperButyl_structu.png 

 

Product attributes

Pore size (mean): 50 nm
Particle size (mean): 100 µm (C-grade)
Pressure rating: 0.3 Mpa
Shipping buffer: 20% ethanol
pH stability: >1-13
Shelf life: 10 years (estimated)

Typical dynamic binding capacities for humanized mAb

HIC_fig2.png 
                                                Binding capacitiy (mg/mL - gel)
                                                (10% breakthrough)
Phenyl-650M                                           35.2
Butyl-650M                                             42.1
PPG-600M                                              44.8
Butyl-600M                                             48.5 

Selectivity of TOYOPEARL HIC resins

HIC_fig3.png
Samples: 1. Ribonuclease A, 2. Lysozyme, 3. α- Chymotrypsinogen

 

Ordering Information

P/N Description Dimension
0019955 TOYOPEARL SuperButyl-550C 25 mL
0019956 TOYOPEARL SuperButyl-550C 100 mL
0019957 TOYOPEARL SuperButyl-550C 1 L
0019958 TOYOPEARL SuperButyl-550C 5 L
0021382 ToyoScreen SuperButyl-550C 6 x 1 mL
0021383 ToyoScreen SuperButyl-550C 6 x 5 mL